Human IL-10 MAb (Clone 23738R) (1 MG)【科瑞奥博】

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Human IL-10 MAb (Clone 23738R) (1 MG)

货号： MAB217R-01M 基本售价： 19254.0 元规格： 1 mg

产品信息

概述
货号	MAB217R-01M
简介	Recombinant Monoclonal Antibody
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
应用	Western Blot,Neutralization
目标/特异性	Detects human IL-10 in direct ELISAs.
使用方法	Western Blot: 2 µg/mL Neutralization: Measured by its ability to neutralize IL-10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. At 5 μg/mL, this antibody will neutralize >60% of the bioactivity due to 5 ng/mL Recombinant Human IL-10.
来源	Recombinant Monoclonal Mouse IgG_2B Clone # 23738R
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	E. coli-derived recombinant human IL-10 Ser19-Asn178 Accession # P22301
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
背景	Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Development and Differentiation B Cell Markers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells ICC / IHC Images: IL-10 IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-10/Interferon Family Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Lymphoma Macrophage Activation Markers Monocyte Markers Mouse Obesity Antibody Array Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Neuroinflammation Plasmacytoid Dendritic Cells Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells Th22 Cells Th9 Cells

参考图片

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217R). At 5 μg/mL, this antibody will neutralize 60% Recombinant Human IL-10 induced bioactivity.