Human FGF R1 (IIIb) MAb (Clone 133111) (500 UG)【科瑞奥博】

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Human FGF R1 (IIIb) MAb (Clone 133111) (500 UG)

货号： MAB765-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB765-500
别名	basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGFBR; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2
全称	Fibroblast Growth Factor Receptor 1
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects IIIb isoforms of human FGF R1. In direct ELISAs and Western blots, this antibody detected IIIb but not IIIc isoforms of human FGF R1, human FGF R2, and mouse FGF R2. In direct ELISA, approximately 20% cross-reactivity was observed with recombinant human (rh) FGF R3 (IIIb). No cross-reactivity was observed with rhFGF R4 or with IIIIc isoforms of rhFGF R1, rhFGF R2, rmFGF R2, or rhFGF R3.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize FGF R1 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.075-0.3 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R1 alpha (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
来源	Monoclonal Mouse IgG₁ Clone # 133111
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2260 (Human); 14182 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I Stem Cells, 2012;30(7):1455-64. Species: Human Sample Type: Whole Cells Application: Neut Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase. Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM J. Biol. Chem., 2009;284(24):16693-703. Species: Human Sample Type: Whole Cells Application: Bioassay
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	A mixture of purified isoforms of recombinant human FGF R1 extracellular domains
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	FGF R1 occurs as multiple isoforms. The extracellular domain consistsof three ( alpha isoforms) or two ( beta isoforms) immunoglobulin-like domains.In addition, the membrane-proximal Ig-like domain can be encoded byalternative exons yielding (IIIb) or (IIIc) isoforms.
运输条件	Blue Ice
存放说明	-20℃
参考文献	FGF Family FGF Ligands FGF Regulators Fibroblast Growth Factor Superfamily Fibroblast Growth Factors and their Receptors Glial Lineage Markers Human CD Marker Products Mini-review: Fibroblast Growth Factors (FGFs) Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway

参考图片

FGF R1 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody. Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).