Human/Mouse IL-5 MAb (Clone TRFK5) (500 UG)【科瑞奥博】

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Human/Mouse IL-5 MAb (Clone TRFK5) (500 UG)

货号： MAB405-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB405-500
别名	BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
全称	Interleukin 5
反应种属	Human/Mouse
应用	CyTOF-reported,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
使用方法	Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.004-0.015 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.
来源	Monoclonal Rat IgG₁ Clone # TRFK5
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice. Authors: Yamada T, Tani Y, Nakanishi H FASEB J., 2011;25(2):561-8. Species: Mouse Sample Type: In Vivo Application: In vivo Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5. Inflamm. Res., 2009;58(2):103-8. Species: Guinea Pig Sample Type: In Vivo Application: In vivo Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Clin. Chem., 2007;53(11):2010-2. Species: Human Sample Type: Plasma Application: ELISA Development Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism. Authors: Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB Clin. Exp. Allergy, 2007;37(1):29-38. Species: Mouse Sample Type: Peritoneal Fluid Application: ELISA Development Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. Authors: Pfleger C, Schloot N, ter Veld F J. Immunol. Methods, 2007;329(1):214-8. Species: Human Sample Type: Serum Application: Luminex Development Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL Am. J. Pathol., 2006;168(6):1940-50. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node. Authors: Nygaard UC, Ormstad H, Aase A, Lovik M Toxicology, 2005;206(2):181-93. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Arthropod-derived histamine-binding protein prevents murine allergic asthma. Authors: Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W J. Immunol., 2004;173(5):3281-6. Species: Mouse Sample Type: BALF Application: ELISA Development Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice. Authors: Kobayashi T, Iijima K, Kita H J. Immunol., 2003;170(11):5756-63. Species: Mouse Sample Type: In Vivo Application: In vivo Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen. Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J Pulm Pharmacol Ther, 2002;15(2):161-8. Species: Mouse Sample Type: Application: In vivo AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ Am. J. Pathol., 2002;160(4):1353-60. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse T cell clone-derived partially purified recombinant mouse IL-5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Common beta Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells Granulocyte Markers Group 2 ILCs ICC / IHC Images: IL-5 Inflammatory Mediators Mini-review: Interleukin 5 T Cell Cytokine Signaling Th2 Cells

参考图片

Detection of IL‑5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab)₂ Secondary Antibody (Catalog # F0105B) and Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody. Recombinant Mouse IL‑5 (Catalog # 405-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL‑5 Monoclonal Antibody (Catalog # MAB405). The ND₅₀ is typically
0.004-0.015 µg/mL.