Mouse IL-6 MAb (Clone MP5-20F3) (500 UG)【科瑞奥博】

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Mouse IL-6 MAb (Clone MP5-20F3) (500 UG)

货号： MAB406-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB406-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB406；Detection antibody：BAF406; and protein: 406-ML-005
别名	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
全称	Interleukin 6
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL‑6, recombinant porcine IL‑6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND₅₀) is typically 0.005-0.025 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse IL‑6.
来源	Monoclonal Rat IgG₁ Clone # MP5-20F3
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity Sci Rep, 2016;6(0):29994. Species: Mouse Sample Type: Whole Cells Application: Neut Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Authors: Jung-Yeon Lim Sci Rep, 2016;6(0):26851. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Capture Oxidative stress-induced inhibition of Sirt1 by caveolin-1 promotes p53-dependent premature senescence and stimulates the secretion of interleukin 6 (IL-6). Authors: Volonte D, Zou H, Bartholomew J, Liu Z, Morel P, Galbiati F J Biol Chem, 2015;290(7):4202-14. Species: Moiuse Sample Type: Cell Lysates Application: WB Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling. Authors: Chandler, Ronald L, Damrauer, Jeffrey, Raab, Jesse R, Schisler, Jonathan, Wilkerson, Matthew, Didion, John P, Starmer, Joshua, Serber, Daniel, Yee, Della, Xiong, Jessie, Darr, David B, Pardo-Manuel de Villena, Fernando, Kim, William, Magnuson, Terry Nat Commun, 2015;6(0):6118. Species: Mouse Sample Type: Whole Cells Application: Neut Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis. Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M Nature, 2015;515(7525):130-3. Species: Mouse Sample Type: In Vivo Application: In vivo KIM-1-mediated phagocytosis reduces acute injury to the kidney. Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J J Clin Invest, 2015;125(4):1620-36. Species: Mouse Sample Type: Cell Lysates Application: ELISA Capture Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F EMBO Mol Med, 2015;7(2):211-26. Species: Human Sample Type: Whole Tissue Application: IHC - Not specified Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Nat Med, 2014;20(4):377-84. Species: Mouse Sample Type: Whole Cells Application: Bioassay Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta. Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y Am J Physiol Endocrinol Metab, 2014;307(2):E186-98. Species: Mouse Sample Type: In Vivo Application: In vivo Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels. Authors: Ke F, Zhang L, Liu Z, Liu J, Yan S, Xu Z, Bai J, Zhu H, Lou F, Wang H, Shi Y, Jiang Y, Su B, Wang H Stem Cells, 2014;32(10):2799-810. Species: Mouse Sample Type: Whole Cells Application: IHC Paraffin-embedded Basophils control T-cell responses and limit disease activity in experimental murine colitis. Authors: Gomez M, Talke Y, Hofmann C, Ketelsen I, Hermann F, Reich B, Goebel N, Schmidbauer K, Dunger N, Bruhl H, Renner K, Syed S, Mack M Mucosal Immunol, 2014;7(1):188-99. Species: Mouse Sample Type: Whole Cells Application: Neut Maternal immune activation during gestation interacts with Disc1 point mutation to exacerbate schizophrenia-related behaviors in mice. Authors: Lipina T, Zai C, Hlousek D, Roder J, Wong A J Neurosci, 2013;33(18):7654-66. Species: Mouse Sample Type: In Vivo Application: In vivo injection Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus. Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C Infect. Immun., 2012;80(1):410-7. Species: Mouse Sample Type: Whole Cells Application: Neut Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. Authors: Roy M, Richard JF, Dumas A, Vallieres L J Neuroinflammation, 2012;9(0):18. Species: Mouse Sample Type: Whole Cells Application: Neut Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy. Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L J. Exp. Med., 2011;208(3):491-503. Species: Mouse Sample Type: Whole Cells Application: Neut Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis. Authors: Osuchowski MF, Connett J, Welch K, Granger J, Remick DG Crit. Care Med., 2009;37(5):1567-73. Species: Mouse Sample Type: Plasma Application: ELISA Development ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T Microbes Infect., 2009;11(4):524-30. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T Immunology, 2008;125(2):272-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening. Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM Bone, 2007;40(5):1276-83. Species: Mouse Sample Type: Whole Cells Application: Neut Maternal immune activation alters fetal brain development through interleukin-6. Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH J. Neurosci., 2007;27(40):10695-702. Species: Mouse Sample Type: In Vivo Application: In vivo Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model. Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M J. Mol. Cell. Cardiol., 2006;41(5):885-92. Species: Mouse Sample Type: Whole Tissue Application: IHC Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH J Ethnopharmacol, 2006;106(3):418-24. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Role of interleukin-6 in cardiomyocyte/cardiac fibroblast interactions during myocyte hypertrophy and fibroblast proliferation. Authors: Fredj S, Bescond J, Louault C, Delwail A, Potreau D J. Cell. Physiol., 2005;204(2):428-36. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Selective macrophage suppression during sepsis. Authors: Ellaban E, Bolgos G, Remick D Cell. Immunol., 2004;231(1):103-11. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways. Authors: Miyamoto M, Prause O, Sjostrand M, Laan M, Lotvall J, Linden A J. Immunol., 2003;170(9):4665-72. Species: Mouse Sample Type: In Vivo Application: In vivo Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes. Authors: Pechova J, StÃ„â€¢pÃƒÂ¡novÃƒÂ¡ G, Kovar L, Kopecky J Folia Parasitol., 2002;49(2):153-9. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature mouse IL-6 is 187 amino acids (aa) in length and shares 42% and 85% aa sequence identity with human and rat IL-6, respectively (5). Alternate splicing generates several isoforms with internal deletions (6). Mouse IL-6 is equally active on rat cells (7). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (8). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (9). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (9). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 10).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. Jones, S.A. (2005) J. Immunol. 175:3468. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193. Yatsenko, O.P. et al. (2004) Cytokine 28:190. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. 85:7099. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

参考图片

Cell Proliferation Induced by IL‑6 and Neutralization by Mouse IL‑6 Antibody.

Recombinant Mouse IL‑6 (Catalog # 406-ML) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑6 Monoclonal Antibody (Catalog # MAB406). The ND₅₀ is typically 0.005-0.025 µg/mL.