Mouse IL-10 MAb (Clone JES052A5) (500 UG)【科瑞奥博】

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Mouse IL-10 MAb (Clone JES052A5) (500 UG)

货号： MAB417-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB417-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB417；Detection antibody：BAF417; and protein: 417-ML-005
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL‑10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL‑10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.005-0.015 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.
来源	Monoclonal Rat IgG₁ Clone # JES052A5
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Authors: Jung-Yeon Lim Sci Rep, 2016;6(0):26851. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Capture IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells. Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F PLoS Pathog, 2015;11(2):e1004607. Species: Mouse Sample Type: In Vivo Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P J Immunol, 2015;194(1):334-48. Species: Mouse Sample Type: Serum Application: ELISA Development Delayed wound healing due to increased interleukin-10 expression in mice with lymphatic dysfunction. Authors: Kimura T, Sugaya M, Blauvelt A, Okochi H, Sato S J Leukoc Biol, 2013;94(1):137-45. Species: Mouse Sample Type: tissue Application: IHC Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer. Authors: Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H PLoS ONE, 2012;7(7):e39525. Species: Mouse Sample Type: In Vivo Application: In vivo Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade. Authors: Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML J. Immunol., 2010;184(9):5360-7. Species: Mouse Sample Type: In Vivo Application: In vivo Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis. Authors: Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y Immunol. Lett., 2009;122(2):198-207. Species: Mouse Sample Type: In Vivo Application: In vivo ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T Microbes Infect., 2009;11(4):524-30. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells. Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N J. Exp. Med., 2008;205(11):2643-55. Species: Mouse Sample Type: In Vivo Application: In vivo CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T Immunology, 2008;125(2):272-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper. Authors: Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D Blood, 2007;110(1):211-9. Species: Mouse Sample Type: Whole Cells Application: Neut Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis. Authors: Ismail N, Crossley EC, Stevenson HL, Walker DH Infect. Immun., 2007;75(9):4608-20. Species: Mouse Sample Type: In Vivo Application: In vivo Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Infect. Immun., 2006;74(12):6730-8. Species: Human Sample Type: BALF Application: ELISA Development Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL Am. J. Pathol., 2006;168(6):1940-50. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Nonlymphoid peritoneal cells suppress the T cell response to Mls. Authors: Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE Immunobiology, 2004;209(8):575-84. Species: Mouse Sample Type: Whole Cells Application: Neut Selective macrophage suppression during sepsis. Authors: Ellaban E, Bolgos G, Remick D Cell. Immunol., 2004;231(1):103-11. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine. Authors: Bednarski JJ, Warner RE, Rao T Arthritis Rheum., 2003;48(3):757-66. Species: Mouse Sample Type: Whole Cells Application: ELISpot Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse IL-10 Accession # P18893
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Development and Differentiation B Cell Markers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells ICC / IHC Images: IL-10 IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-10/Interferon Family Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Lymphoma Macrophage Activation Markers Monocyte Markers Mouse Obesity Antibody Array Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Neuroinflammation Plasmacytoid Dendritic Cells Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells Th22 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417). The ND₅₀ is typically 0.005-0.015 µg/mL.