Human IFN-gamma R1 MAb (Clone GIR208) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human IFN-gamma R1 MAb (Clone GIR208) (500 UG)

货号： MAB6732-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB6732-500
别名	AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma receptor 1; IFN-gamma-R1; IFNGR; IFNGR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2
全称	Interferon gamma Receptor 1
反应种属	Human
应用	Neutralization
目标/特异性	Detects human IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta is observed.
使用方法	Neutralization: Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.03-0.15 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.
来源	Monoclonal Mouse IgG₁ Clone # GIR208
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3459 (Human); 15979 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Chronic lymphocytic leukemia cells express CD38 in response to Th1 cell-derived IFN-gamma by a T-bet-dependent mechanism. Authors: Burgler S, Gimeno A, Parente-Ribes A, Wang D, Os A, Devereux S, Jebsen P, Bogen B, Tjonnfjord G, Munthe L J Immunol, 2015;194(2):827-35. Species: Mouse Sample Type: In Vivo Application: In vivo IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients. Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S J Allergy Clin Immunol, 2013;131(2):562-70. Species: Human Sample Type: whole cells Application: Blocking A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G J. Leukoc. Biol., 2001;70(4):617-23. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Human placenta-derived IFN‑ gamma R1/CD119
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% aa identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Bach, E.A.et al. (1997) Annu. Rev. Immunol. 15:563.

参考图片

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose‑dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN‑ gamma R1/CD119 Monoclonal Antibody (Catalog # MAB6732). The ND₅₀ is typically 0.03‑0.15 µg/mL.