| 货号 | MAB6843-500 |
| 别名 | BFR-1; CD332 antigen; CD332; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KSAM; K-sam; soluble FGFR4 variant 4 | 全称 | Fibroblast Growth Factor Receptor 2 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(8-25 µg/mL) |
| 目标/特异性 | Detects all isoforms of human FGF R2 ( alpha and beta, IIIb and IIIc). In direct ELISAs, approximately 25‑50% cross-reactivity with mouse FGF R2 and no cross-reactivity with any isoform of recombinant human (rh) FGF R1, rhFGF R3, or rhFGF R4 is observed. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize FGF R2 beta -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R2 beta (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin. |
| 来源 | Monoclonal Mouse IgG1 Clone # 98739 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2263 (Human); 14183 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Fibroblast Growth Factor Receptor 2 (FGF R2) is one of four closely related transmembrane tyrosine kinases (FGF R1‑4) that function as receptors for the fibroblast growth factor family. Multiple isoforms are generated by alternative mRNA splicing resulting in extracellular domains with three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. In addition, alternative exon usage in the Ig III (membrane proximal) domain results in IIIb or IIIc isoforms. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 beta (IIIc) Fc Chimera (Catalog # 684-FR) inhibits Recombinant Human FGF acidic (Catalog # 232‑FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 beta (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB6843). The ND50 is typically 0.1‑0.5 µg/mL in the presence of heparin (10 µg/mL). | |
FGF R2 in Human Adenocarcinoma. FGF R2 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human FGF R2 Monoclonal Antibody (Catalog # MAB6843) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
