Human FGF R2 MAb (Clone 98725) (500 UG)【科瑞奥博】

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Human FGF R2 MAb (Clone 98725) (500 UG)

货号： MAB684-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB684-500
别名	BFR-1; CD332 antigen; CD332; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC 2.7.10.1; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KSAM; K-sam; soluble FGFR4 variant 4
全称	Fibroblast Growth Factor Receptor 2
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects alpha isoforms (3 Ig-like domains) and beta isoforms (2 Ig-like domains) of human FGF R2 in direct ELISAs and Western blots. In direct ELISAs, this antibody reacts with all isoforms of rhFGF R2 but shows approximately a 4-fold preference for (IIIc) isoforms. Approximately 100% cross-reactivity with recombinant mouse FGF R2, 15% cross-reactivity with recombinant human (rh) FGF RI ( beta isoforms), and no cross-reactivity with rhFGF RI ( alpha isoforms), rhFGF R3, or rhFGF R4 is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize FGF R2 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 4 ng/mL Recombinant Human FGF R2 alpha (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
来源	Monoclonal Mouse IgG₁ Clone # 98725
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2263 (Human); 14183 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J Stem Cells, 2007;25(7):1737-45. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha isoforms) or two ( beta isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.
运输条件	Blue Ice
存放说明	-20℃
参考文献	FGF Family FGF Ligands FGF Regulators Fibroblast Growth Factor Superfamily Fibroblast Growth Factors and their Receptors Glial Lineage Markers Human CD Marker Products ICC / IHC Images: FGF R2 Mesenchymal to Epithelial Transition Mini-review: Fibroblast Growth Factors (FGFs) Neural Stem Cell Markers Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway

参考图片

FGF R2 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R2 Antibody. Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (Catalog # 712-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R2 alpha (IIIc) Fc Chimera (4 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R2 Monoclonal Antibody (Catalog # MAB684). The ND₅₀ is typically 1-5 µg/mL in the presence of heparin (10 µg/mL).