Human LIF MAb (Clone 9824) (500 UG)【科瑞奥博】

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Human LIF MAb (Clone 9824) (500 UG)

货号： MAB250-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB250-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB250；Detection antibody：BAF250
别名	CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; Melanoma-derived LPL inhibitor; MLPLI
全称	Leukemia Inhibitory Factor
反应种属	Human
应用	Immunohistochemistry(8-25 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
目标/特异性	Detects human LIF in ELISAs and Western blots. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhOSM, rhTGF-beta 1, rhTNF-alpha, rhTNF-beta, recombinant mouse (rm) IL-1 beta, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmGM-CSF, bovine (b) FGF acidic, bFGF basic, human (h) PDGF, porcine (p) PDGF, hTGF-beta 1, pTGF-beta 1.2, or pTGF-beta 2 is observed.
使用方法	Immunohistochemistry: 8-25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL Neutralization: Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.06-0.2 µg/mL in the presence of 1.5 ng/mL Recombinant Human LIF. Human LIF Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) is recommended for neutralization.
来源	Monoclonal Mouse IgG_2B Clone # 9824
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3976 (Human); 16878 (Mouse); 403449 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS. Authors: Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C J. Biol. Chem., 2011;286(39):34271-85. Species: Human Sample Type: Cell Culture Supernates Application: WB Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Blood, 2007;110(13):4319-30. Species: Human Sample Type: Cell Culture Supernates Application: Neut Immunocytochemical detection and RT-PCR expression of leukaemia inhibitory factor and its receptor in human fetal and adult ovaries. Authors: Abir R, Fisch B, Jin S, Barnnet M, Freimann S, van den Hurk R, Feldberg D, Nitke S, Krissi H, Ao A Mol. Hum. Reprod., 2004;10(5):313-9. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes. Authors: Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A Arthritis Rheum., 2001;44(10):2392-401. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human LIF
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells (17).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337. Gough, N.M. (1992) Growth Factors 7:175. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973. Gillett, N.A. et al. (1993) Growth Factors 9:301. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260. Williams, R.L. et al. (1988) Nature 336:684. Baumann, H. et al. (1989) J. Immunol. 143:1163. Marshall, M.K. et al. (1994) Endocrinology 135:1412. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283. Davis, S. et al. (1993) Science 260:18054. Gearing, D.P. et al. (1991) EMBO J. 10:28395. Gearing, D.P. et al. (1991) Science 255:1434. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.

参考图片

Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB250). The ND₅₀ is typically 0.06-0.2 µg/mL.