Human ST2/IL-1 R4 MAb (Clone 97203) (500 UG)【科瑞奥博】

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Human ST2/IL-1 R4 MAb (Clone 97203) (500 UG)

货号： MAB523-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB523-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB523-500；Detection antibody：BAF523; and protein: 523-ST-100
别名	DER4; Fit-1; IL-1 R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2; T1
全称	Interleukin 33 Receptor
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human ST2/IL-33 R in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse ST2L, recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 RA, rhIL-1 alpha, or rhIL-1 beta is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC).The Neutralization Dose (ND₅₀) is typically 0.3-1.5 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.
来源	Monoclonal Mouse IgG₁ Clone # 97203
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Daily exposure to dust alters innate immunity. Authors: Sahlander K, Larsson K, Palmberg L PLoS ONE, 2012;7(2):e31646. Species: Human Sample Type: Whole Cells Application: Neut The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis. Authors: Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T Clin. Exp. Allergy, 2012;42(2):218-28. Species: Human Sample Type: Whole Cells Application: Neut IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. Authors: Blom L, Poulsen L J Immunol, 2012;189(9):4331-7. Species: Human Sample Type: Whole Cells Application: Neut Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells. Authors: Yasuo T, Kitaya K Fertil. Steril., 2009;92(2):709-21. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R Lys19-Phe328 Accession # BAA02233
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks the ability of IL‑33 to signal through transmembrane ST2 (10, 13‑15).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31. Tominaga, S.et al. (1992) Biochim. Biophys. Acta 1171:215. Li, H. et al. (2000) Genomics 67:284. Schmitz, J. et al. (2005) Immunity 23:479. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979. Brint, E.K. et al. (2004) Nat. Immunol. 5:373. Weinberg, E.O. et al. (2002) Circulation 106:2961. Sanada S. et al. (2007) J. Clin. Invest. 117:1538. Palmer, G. et al. (2008) Cytokine 42:358. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723. Miller, A.M. et al. (2008) J. Exp. Med. 205:339. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

参考图片

IFN-gamma Secretion Induced by
IL‑33 and Neutralization by Human ST2/IL-33 R Antibody.

Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND₅₀ is typically 0.3-1.5 μg/mL.