TGF-beta 1 MAb (Clone 9016) (500 UG)【科瑞奥博】

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TGF-beta 1 MAb (Clone 9016) (500 UG)

货号： MAB240-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB240-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB240；Detection antibody：BAF240; and protein: 240-B-002
别名	CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1
全称	Transforming Growth Factor beta 1
应用	Western Blot,Immunohistochemistry,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects TGF-beta 1 from human, mouse, rat, and other species in direct ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant human (rh) TGF‑ beta 3 and recombinant amphibian TGF‑ beta 5 and no cross-reactivity with recombinant porcine TGF-beta 2 or rhTGF-beta 2 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND₅₀) is typically 0.3-1.0 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.
来源	Monoclonal Mouse IgG₁ Clone # 9016
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Platelet-Derived Ectosomes Reduce NK Cell Function J Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N J Immunol, 2015;195(5):2141-8. Species: Human Sample Type: Whole Cells Application: Neut Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. Authors: Sommer A, Fabri M PLoS ONE, 2015;10(6):e0130395. Species: Human Sample Type: Whole Cells Application: Neut Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Proc Natl Acad Sci U S A, 2015;112(9):E957-65. Species: Mouse Sample Type: Whole Cells Application: Flow Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-ss Authors: Ogawa H, Ledford J, Mukherjee S, Aono Y, Nishioka Y, Lee J, Izumi K, Hollingsworth J Respir Res, 2014;15(1):143. Species: Mouse Sample Type: In Vivo Application: In vivo Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D J Immunol, 2014;192(2):804-16. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Mesenchymal stem cells cancel azoxymethane-induced tumor initiation. Authors: Nasuno M, Arimura Y, Nagaishi K, Isshiki H, Onodera K, Nakagaki S, Watanabe S, Idogawa M, Yamashita K, Naishiro Y, Adachi Y, Suzuki H, Fujimiya M, Imai K, Shinomura Y Stem Cells, 2014;32(4):913-25. Species: Rat Sample Type: Whole Cells Application: Neut Regulation of renal fibrosis by Smad3 Thr388 phosphorylation. Authors: Qu X, Li X, Zheng Y, Ren Y, Puelles V, Caruana G, Nikolic-Paterson D, Li J Am J Pathol, 2014;184(4):944-52. Species: Rat Sample Type: Whole Cells Application: Neut Exosomes with membrane-associated TGF-beta1 from gene-modified dendritic cells inhibit murine EAE independently of MHC restriction. Authors: Yu L, Yang F, Jiang L, Chen Y, Wang K, Xu F, Wei Y, Cao X, Wang J, Cai Z Eur J Immunol, 2013;43(9):2461-72. Species: Mouse Sample Type: exosomes Application: Neut TGF-beta signaling regulates neuronal C1q expression and developmental synaptic refinement. Authors: Bialas A, Stevens B Nat Neurosci, 2013;16(12):1773-82. Species: Mouse Sample Type: Cell Culture Supernates Application: Immunodepletion Reduction of methylglyoxal-induced glycation by pyridoxamine improves adipose tissue microvascular lesions. Authors: Rodrigues T, Matafome P, Santos-Silva D, Sena C, Seica R J Diabetes Res, 2013;2013(0):690650. Species: Rat Sample Type: tissue Application: IHC Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis. Authors: Zhu Y, Colak T, Shenoy M, Liu L, Mehta K, Pai R, Zou B, Xie X, Pasricha P Mol Pain, 2012;8(0):65. Species: Rat Sample Type: In Vivo Application: Neut HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages. Authors: Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay MJ PLoS ONE, 2012;7(3):e32761. Species: Human Sample Type: recombinant TGF-beta1 Application: Neut Overexpression of TGF-ÃŸ 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ÃŸ. Authors: Oida T, Weiner HL J. Immunol., 2012;185(6):3529-35. Species: Human Sample Type: whole cells Application: Flow Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease. Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y J. Clin. Invest., 2011;121(11):4548-66. Species: Human Sample Type: recombinant TGF-b1 Application: WB C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY Diabetologia, 2011;54(10):2713-23. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ Mol. Biol. Cell, 2011;22(10):1686-98. Species: Canine Sample Type: Whole Cells Application: Neut Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity. Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S J. Biol. Chem., 2011;286(20):17435-44. Species: Mouse Sample Type: Whole Cells Application: Neut AIDS progression is associated with the emergence of IL-17-producing cells early after simian immunodeficiency virus infection. Authors: Campillo-Gimenez L, Cumont MC, Fay M, Kared H, Monceaux V, Diop O, Muller-Trutwin M, Hurtrel B, Levy Y, Zaunders J, Dy M, Leite-de-Moraes MC, Elbim C, Estaquier J J. Immunol., 2010;184(2):984-92. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Tissue Application: IHC Frozen Activation of Toll-like receptor 3 augments myofibroblast differentiation. Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M Am. J. Respir. Cell Mol. Biol., 2009;40(6):654-62. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Loss of STAT5 causes liver fibrosis and cancer development through increased TGF-{beta} and STAT3 activation. Authors: Hosui A, Kimura A, Yamaji D, Zhu BM, Na R, Hennighausen L J. Exp. Med., 2009;206(4):819-31. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection. Authors: Bhowmick S, Mazumdar T, Ali N Infect. Immun., 2009;77(4):1514-23. Species: Mouse Sample Type: In Vivo Application: In vivo Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A Blood, 2009;113(13):2955-64. Species: Human Sample Type: Whole Cells Application: Neut Intrastromal keratotomy with femtosecond laser avoids profibrotic TGF-beta1 induction. Authors: Meltendorf C, Burbach GJ, Ohrloff C, Ghebremedhin E, Deller T Invest. Ophthalmol. Vis. Sci., 2009;50(8):3688-95. Species: Rabbit Sample Type: Whole Tissue Application: IHC Frozen Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells. Authors: Pot C, Jin H, Awasthi A J. Immunol., 2009;183(2):797-801. Species: Mouse Sample Type: Whole Cells Application: Neut Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection. Authors: Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z J. Immunol., 2008;180(1):113-21. Species: Mouse Sample Type: In Vivo Application: In vivo The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH Stem Cells, 2008;26(4):1047-55. Species: Rat Sample Type: Tissue Homogenates Application: ELISA Development Transforming growth factor-beta stimulates intestinal epithelial focal adhesion kinase synthesis via smad- and p38-dependent mechanisms. Authors: Walsh MF, Ampasala DR, Hatfield J, Vander Heide R, Suer S, Rishi AK, Basson MD Am. J. Pathol., 2008;173(2):385-99. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Cell Lysates Application: WB IL-2 is essential for TGF-beta to convert naive CD4+CD25- cells to CD25+Foxp3+ regulatory T cells and for expansion of these cells. Authors: Zheng SG, Wang J, Wang P, Gray JD, Horwitz DA J. Immunol., 2007;178(4):2018-27. Species: Mouse Sample Type: Whole Cells Application: Neut Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Authors: Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Nat. Immunol., 2007;8(9):942-9. Species: Human Sample Type: Whole Cells Application: Neut Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells. Authors: Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ, Yokota SJ, Bankert RB J. Immunol., 2007;178(9):5552-62. Species: Human Sample Type: Whole Cells Application: Neut A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J Blood, 2007;109(5):2234-42. Species: Mouse Sample Type: Cell Culture Supernates Application: Array Development Inhibition of p38 mitogen-activated protein kinase and transforming growth factor-beta1/Smad signaling pathways modulates the development of fibrosis in adriamycin-induced nephropathy. Authors: Li J, Campanale NV, Liang RJ, Deane JA, Bertram JF, Ricardo SD Am. J. Pathol., 2006;169(5):1527-40. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression. Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY Immunol. Lett., 2006;105(2):159-66. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Neut Increased frequency of CD4+ cells expressing CD161 in cancer patients. Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA Clin. Cancer Res., 2006;12(23):6901-9. Species: Human Sample Type: Whole Cells Application: Neut Increase of CD4+ CD25+ regulatory T-cells in the liver of patients with hepatocellular carcinoma. Authors: Yang XH, Yamagiwa S, Ichida T, Matsuda Y, Sugahara S, Watanabe H, Sato Y, Abo T, Horwitz DA, Aoyagi Y J. Hepatol., 2006;45(2):254-62. Species: Human Sample Type: Whole Cells Application: Neut Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Authors: Phillips TM Electrophoresis, 2004;25(10):1652-9. Species: Human Sample Type: Serum Application: Affinity Chromatography trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. Authors: Lim HW, Hillsamer P, Kim CH J. Clin. Invest., 2004;114(11):1640-9. Species: Human Sample Type: Whole Cells Application: Neut Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Induction of a hypertrophic growth status of coronary smooth muscle cells is associated with an overexpression of TGF-beta. Authors: Schmidt A, Gopfert C, Vlodavsky I, Volker W, Buddecke E Eur. J. Cell Biol., 2002;81(3):138-44. Species: Bovine Sample Type: Whole Cells Application: Neut Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription. Authors: Jono H, Shuto T, Xu H, Kai H, Lim DJ, Gum JR, Kim YS, Yamaoka S, Feng XH, Li JD J. Biol. Chem., 2002;277(47):45547-57. Species: Human Sample Type: Cell Lysates Application: Neut Repeated exposure induces periportal fibrosis in Schistosoma mansoni-infected baboons: role of TGF-beta and IL-4. Authors: Farah IO, Mola PW, Kariuki TM, Nyindo M, Blanton RE, King CL J. Immunol., 2000;164(10):5337-43. Species: Primate - Papio anubis (Olive Baboon) Sample Type: Cell Culture Supernates Application: ELISA Development Isoform specificity of commercially-available anti-TGF-beta antibodies. Authors: Mozes MM, Hodics T, Kopp JB J. Immunol. Methods, 1999;225(1):87-93. Species: Human Sample Type: recombinant TGFb1 Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant TGF‑ beta 1 and latent TGF‑ beta 1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TGF-beta 1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2, and -3 are disulfide-linked homodimers.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Airway Inflammation and Remodeling Cancer Biomarkers Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Early Mesodermal Lineage Markers EMT Induction HIF Transcription Factors ICC / IHC Images: TGF-beta 1 Inflammatory Mediators Myeloid-derived Suppressor Cells (MDSC) Platelets Preeclampsia Regulatory T Cells (Tregs) TGF-beta Family TGF-beta Family Ligands

参考图片

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1 Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240). The ND₅₀ is typically 0.3-1.0 µg/mL.

Detection of TGF‑ beta 1 in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab)₂ Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

TGF-beta 1 in Human Prostate Cancer Tissue. TGF-beta 1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-
TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB240) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in the prostate gland. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.