| 货号 | MAB2601-500 |
| 别名 | cardiophin 1; cardiotrophin 1; cardiotrophin-1; CT-1CT1; CTF1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 20 ng/mL Recombinant Human Cardiotrophin‑1/CT‑1. |
| 来源 | Monoclonal Mouse IgG1 Clone # 89215 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1489 (Human); 13019 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human Cardiotrophin‑1/CT‑1 Ser2-Ala201 Accession # Q16619 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody. Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin‑1/CT‑1 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Cardiotrophin‑1/CT‑1 Mono- clonal Antibody (Catalog # MAB2601). The ND50 is typically 1‑3 µg/mL. |
