Human VEGF R2/KDR MAb (Clone 89106) (500 UG)【科瑞奥博】

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Human VEGF R2/KDR MAb (Clone 89106) (500 UG)

货号： MAB3572-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB3572-500
别名	CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGFR; VEGFR2; VEGFR-2
全称	Vascular Endothelial Growth Factor Receptor 2
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 10-50 ng/mL in the presence of 50 ng/mL Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Human VEGF₁₆₅.
来源	Monoclonal Mouse IgG₁ Clone # 89106
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Nature, 2011;475(7355):226-30. Species: Human Sample Type: Whole Cells Application: Neut Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt. Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7. Species: Human Sample Type: Whole Cells Application: Neut Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells. Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T Biochem. Biophys. Res. Commun., 2008;368(3):543-9. Species: Human Sample Type: Whole Cells Application: Neut Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels. Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U Leuk. Res., 2008;32(8):1244-58. Species: Human Sample Type: Whole Cells Application: Flow Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Whole Cells Application: Flow Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Stem Cells, 2007;25(2):490-9. Species: Human Sample Type: Whole Cells Application: Flow Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U Stem Cells, 2007;25(8):1862-71. Species: Human Sample Type: Whole Cells Application: Flow Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. Authors: Lin YL, Liang YC, Chiang BL J. Leukoc. Biol., 2007;82(6):1473-80. Species: Human Sample Type: Whole Cells Application: Neut Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis. Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA Blood, 2007;109(4):1479-89. Species: Human Sample Type: Whole Cells Application: Neut Thiol regulation of vascular endothelial growth factor-A and its receptors in human retinal pigment epithelial cells. Authors: Sreekumar PG, Kannan R, de Silva AT, Burton R, Ryan SJ, Hinton DR Biochem. Biophys. Res. Commun., 2006;346(4):1200-6. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1 Ala20-Glu764 Accession # P35968
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

参考图片

Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

VEGF R2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGF R2/KDR Antibody. Recombinant Human VEGF R2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog #293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅ (5 ng/mL) activity elicited by Recombinant Human VEGF R2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGF R2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND₅₀ is typically 10-50 ng/mL.