Human CXCL11/I-TAC MAb (Clone 87328) (500 UG)【科瑞奥博】

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Human CXCL11/I-TAC MAb (Clone 87328) (500 UG)

货号： MAB672-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB672-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB672；Detection antibody：BAF672; and protein: 672-IT-025
别名	beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human CXCL11/I-TAC in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL5, 7, 8, 9, or 10. In Western blots, this antibody shows less than 1% cross-reactivity with rhCXCL1, 2, 3, 5, 6, 7, IL-8/CXCL8, 9, 10, 13, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, rmCXCL1, 2, 6, 9, 10, CXCL12/SDF-1 alpha, rmCXCL13, rrCXCL1, CXCL3/CINC-2 alpha, CXCL3/CINC-2 beta, or rpIL-8/CXCL8.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 15 ng/mL Recombinant Human CXCL11/I‑TAC.
来源	Monoclonal Mouse IgG_2A Clone # 87328
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6373 (Human); 56066 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH J. Immunol., 2010;184(6):2886-98. Species: Human Sample Type: Whole Cells Application: Neut Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D J. Immunol., 2007;179(6):4272-82. Species: Human Sample Type: Serum Application: Immunodepletion CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow. Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH Am. J. Pathol., 2005;167(3):887-99. Species: Human Sample Type: Tissue Homogenates Application: WB Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Authors: Liu L, Jarjour NN, Busse WW, Kelly EA Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24. Species: Human Sample Type: BALF Application: ELISA Development Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D J. Immunol., 2004;173(11):6592-602. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CXCL11/I-TAC Phe22-Phe94 Accession # O14625
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cole, K. et al. (1998) J. Exp. Med. 187:2009. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878. Lou, Y. et al. (1998) J. Neurovirol. 4:575.

参考图片

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody. Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND₅₀ is typically 0.2-0.8 µg/mL.