Human CX3CL1/Fractalkine MAb (Clone 81506) (500 UG)【科瑞奥博】

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Human CX3CL1/Fractalkine MAb (Clone 81506) (500 UG)

货号： MAB3652-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB3652-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB3652；Detection antibody：BAM365; and protein: 365-FR-025
别名	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1
反应种属	Human
应用	CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.
来源	Monoclonal Mouse IgG₁ Clone # 81506
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C Mucosal Immunol, 2012;5(0):702. Species: Human Sample Type: Whole Cells Application: Flow The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells. Authors: Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP Cancer Lett., 2009;273(1):127-39. Species: Human Sample Type: Whole Cells Application: Flow Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. Authors: Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T J. Leukoc. Biol., 2008;84(1):215-23. Species: Human Sample Type: Cell Culture Supernates Application: WB Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A J. Immunol., 2007;178(12):8064-72. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Fractalkine mediates natural killer-dependent antitumor responses in vivo. Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C Cancer Res., 2003;63(21):7468-74. Species: Human Sample Type: Whole Cells Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0-derived and E. coli-derived Recombinant Human CX3CL1/Fracktalkine Gln25-Arg339 Accession # P78423
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CX3CL1, also known as Fractalkine, is a type I membrane protein inwhich a chemokine domain possessing a unique C-X3-C cysteine motif istethered on a long mucin-like stalk. It can also be released as asoluble molecule upon proteolysis at a membrane proximal site.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Stem Cell Markers Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CX3C Chemokines and Receptors Fractalkine in Glomerulonephritis Fractalkine Signaling Modulates Neurotoxicity in vivo Glomerulonephritis: Beyond Inflammation ICC / IHC Images: CX3CL1/Fractalkine Macrophage Activation Markers Neuroinflammation Renal Markers Special Glycoproteins

参考图片

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody. Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND₅₀ is typically 1-5 µg/mL.