Porcine IL-1 beta/IL-1F2 MAb (Clone 77724) (500 UG)【科瑞奥博】

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Porcine IL-1 beta/IL-1F2 MAb (Clone 77724) (500 UG)

货号： MAB6811-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB6811-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB6811-500；Detection antibody：BAF681; and protein: 681-PI-010
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Porcine
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects porcine IL-1 beta in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) IL‑1 beta and recombinant mouse (rm) IL‑1 beta is observed and no cross-reactivity with rhIL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha or recombinant rat IL-1 alpha is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND₅₀) is typically 4-15 µg/mL in the presence of 75 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Monoclonal Mouse IgG₁ Clone # 77724
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate. Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK J. Virol., 2006;80(21):10514-21. Species: Porcine Sample Type: Cell Lysates Application: WB Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Cytometry A, 2006;69(5):391-5. Species: Porcine Sample Type: Serum Application: Luminex Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant porcine IL‑1 beta /IL-1F2 Ala115-Pro267 Accession # P26889.
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Porcine
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. Huether, M. et al. (1993) Gene 129:285. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

参考图片

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine
IL‑1 beta /IL‑1F2 Antibody. Recombinant Porcine
IL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine
IL‑1 beta /IL‑1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811). The ND₅₀ is typically 4-15 µg/mL in the presence of concanavalin A (1.25 µg/mL).