| 货号 | MAB5334-500 |
| 别名 | EC 3.2.1; EC 3.2.1.31; KL; klotho |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human Klotho in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Klotho (aa 35-982) is observed and no cross-reactivity with rmKlotho (aa 23-550), recombinant human (rh) Klotho beta (aa 53-997), or rhCytosolic beta‑Glucosidase/GBA3 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize Klotho-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human FGF RIIIc. The Neutralization Dose (ND50) is typically 0.15-0.6 ug/mL in the presence of 0.3 ug/mL Recombinant Human Klotho. |
| 来源 | Monoclonal Rat IgG2B Clone # 775340 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9365 (Human); 16591 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Klotho Glu34-Ser981 Accession # Q9UEF7 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1012 amino acid (aa) type I transmembrane protein contains a 32 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, human Klotho shares 87%, 90%, 90% and 86% aa identity with mouse, rat, bovine and equine Klotho, respectively. Although a truncated 549 aa isoform predicts a soluble 70 kDa form, the form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho‑deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate‑limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D)(7). Klotho‑deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by Klotho and Neutralization by Human Klotho Antibody. Recombinant Human Klotho (Catalog # 5334-KL) induces proliferation in the BaF3 mouse pro‑B cell line transfected with human FGF RIIIc in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Klotho (0.3 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human Klotho Monoclonal Antibody (Catalog # MAB5334). The ND50 is typically 0.15-0.6 μg/mL. |
