| 货号 | MAB7346-500 |
| 别名 | BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2 | 全称 | Transforming Growth Factor beta 2 |
| 反应种属 | Mouse |
| 应用 | Neutralization |
| 目标/特异性 | Detects mouse TGF‑ beta 2 in direct ELISAs.In direct ELISAs, approximately 50%cross-reactivity with recombinant human (rh) TGF-beta 2 and rhTGF-beta 3 isobserved. |
| 使用方法 | Neutralization: Measured by its ability to neutralize TGF‑ beta 2 inhibition of IL-4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 5 ng/mL Recombinant Mouse TGF‑ beta 2 and 7.5 ng/mL Recombinant Mouse IL-4. |
| 来源 | Monoclonal Rat IgG2B Clone # 771213 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7042 (Human); 21808 (Mouse); 397084 (Porcine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse TGF‑ beta 2 Ala303-Ser414 Accession # P27090 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | TGF-beta 2(transforming growth factor beta 2) is one of three closely related mammalianmembers of the large TGF-beta superfamily that share a characteristic cysteineknot structure. TGF-beta 1, -2 and -3 are highly pleiotropiccytokines proposed to act as cellular switches that regulate processessuch as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; forTGF-beta 2, mice with targeted deletion show defects in development of cardiac,lung, craniofacial, limb, eye, ear and urogenital systems. Mouse TGF-beta 2cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signalpeptide and a 395 aa proprotein. A furin-like convertase processes theproprotein to generate an N-terminal 283 aa latency-associated peptide (LAP)and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimersof LAP and TGF-beta 2 remain non-covalently associated after secretion, forming thesmall latent TGF-beta 2 complex. Covalent linkage of LAP to one of threelatent TGF-beta binding proteins (LTBPs) creates a large latent complex that mayinteract with the extracellular matrix. TGF-beta is activated fromlatency by pathways that include actions of the protease plasmin, matrixmetalloproteases, thrombospondin 1 and a subset of integrins. Mature mouseTGF-beta 2 shares 100% aa identity with rat TGF-beta 2, and 97% aa identity withhuman, porcine, canine, equine and bovine TGF-beta 2. It demonstratescross-species activity. In most cells, TGF-beta 2 signaling begins with bindingto a complex of the accessory receptor betaglycan (also known asTGF-beta RIII) and a type II ser/thr kinase receptor termed TGF-beta RII,which then phosphorylates and activates another ser/thr kinase receptor,TGF-beta RI (also called activin receptor-like kinase (ALK) -5), oralternatively, ALK-1. The whole complex phosphorylates and activates Smadproteins that regulate transcription. In bone -related cells,however, TGF-beta 2 also signals through TGF-beta RIIB (a splice variant of TGF-beta RII), independently of TGF-beta RIII. Use of other signaling pathwaysthat are Smad-independent allows for disparate actions observed in response toTGF-beta in different contexts. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
TGF‑ beta 2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by Mouse TGF‑ beta 2 Antibody. Recombinant Mouse TGF‑ beta 2 (Catalog # 7346‑B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Mouse TGF-beta 2 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse TGF‑ beta 2 Monoclonal Antibody (Catalog # MAB7346). The ND50 is typically 0.15-0.75 ug/mL. |
