Human TRAIL/TNFSF10 MAb (Clone 75411) (500 UG)【科瑞奥博】

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Human TRAIL/TNFSF10 MAb (Clone 75411) (500 UG)

货号： MAB375-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB375-500
别名	Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10
全称	TNF-related Apoptosis-inducing Ligand
反应种属	Human
应用	Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human TRAIL/TNFSF10 in direct ELISAs.
使用方法	Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 2-8 ng/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody, and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 75411
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8743 (Human); 22035 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Negative control of TRAIL-R1 signaling by transforming growth factor ?1 in pancreatic tumor cells involves Smad-dependent down regulation of TRAIL-R1 Cell Signal, 2016;28(11):1652-1662. Species: Human Sample Type: Whole Cells Application: Neut Human plasmacytoid dendritic cells are equipped with antigen-presenting and tumoricidal capacities. Authors: Tel J, Smits E, Anguille S, Joshi R, Figdor C, de Vries I Blood, 2012;120(19):3936-44. Species: Human Sample Type: whole cells Application: Bioassay TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity. Authors: Kalb ML, Glaser A, Stary G, Koszik F, Stingl G J. Immunol., 2012;188(4):1583-91. Species: Human Sample Type: Whole Cells Application: Neut TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN. Authors: Inglefield JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP J. Interferon Cytokine Res., 2008;28(4):253-63. Species: Human Sample Type: Whole Cells Application: Neut TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor. Authors: Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR J. Biol. Chem., 2008;283(12):7754-62. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells. Authors: Krysov SV, Krysov</LastName><ForeNam SV</Initia, Rowley TF, Al-Shamkhani A Mol. Cancer Ther., 2007;6(2):703-11. Species: Human Sample Type: Whole Cells Application: Neut A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK Cell Death Differ., 2006;13(10):1789-801. Species: Human Sample Type: Whole Cells Application: Neut Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D J. Leukoc. Biol., 2005;78(2):359-71. Species: Human Sample Type: Whole Cells Application: Neut Adenoviral gene transfer of tumor necrosis factor-related apoptosis-inducing ligand overcomes an impaired response of hepatoma cells but causes severe apoptosis in primary human hepatocytes. Authors: Armeanu S, Lauer UM, Smirnow I, Schenk M, Weiss TS, Gregor M, Bitzer M Cancer Res., 2003;63(10):2369-72. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10 Thr95-Gly281 Accession # P50591
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TRAIL is a type II transmembrane protein belonging to the TNFsuperfamily and is now designated TNFSF10. TRAIL is active as ahomotrimer and is produced by a variety of cell types in both themembrane bound form and also as a soluble molecule. It binds to any ofthe four TRAIL receptors as well as to Osteoprotegerin.
运输条件	Blue Ice
存放说明	-20℃
参考文献	gamma delta T Cells Human CD Marker Products ICC / IHC Images: TRAIL/TNFSF10 Isolation, Culture, and Identification of Natural Killer Cells Natural Killer T (NKT) Cells Regulation of Apoptosis by TNF Superfamily Members Regulatory T Cells (Tregs) TNF Superfamily TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB375). The ND₅₀ is typically 2-8 ng/mL.