Human IL-18 R alpha/IL-1 R5 MAb (Clone 70625) (500 UG)【科瑞奥博】

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Human IL-18 R alpha/IL-1 R5 MAb (Clone 70625) (500 UG)

货号： MAB840-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB840-500
别名	CD218a; IL-1 R5; IL-1 Rrp; IL-18 R1; IL18R1
全称	Interleukin 18 Receptor alpha
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects Recombinant Human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells. The Neutralization Dose (ND₅₀) is typically 0.03-0.1 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF-alpha.
来源	Monoclonal Mouse IgG₁ Clone # 70625
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8809 (Human); 16174 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 2016;6(0):26757. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. Authors: Blom L, Poulsen L J Immunol, 2012;189(9):4331-7. Species: Human Sample Type: Whole Cells Application: Neut Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Authors: Takei S, Hoshino T, Matsunaga K, Sakazaki Y, Sawada M, Oda H, Takenaka S, Imaoka H, Kinoshita T, Honda S, Ida H, Fukuda TA, Aizawa H Arthritis Res. Ther., 2011;13(2):R52. Species: Human Sample Type: Serum Application: WB Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18. Authors: Tucci M, Quatraro C, Lombardi L, Pellegrino C, Dammacco F, Silvestris F Arthritis Rheum., 2008;58(1):251-62. Species: Human Sample Type: Whole Cells Application: Flow CD molecules 2006--human cell differentiation molecules. Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X J. Immunol. Methods, 2007;319(1):1-5. Species: Human Sample Type: Whole Cells Application: Flow IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. Authors: Rao DA, Tracey KJ, Pober JS J. Immunol., 2007;179(10):6536-46. Species: Human Sample Type: Whole Cells Application: Neut Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5 Glu20-Arg329 Accession # Q13478
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Parnet, P.et al. (1996) J. Biol. Chem. 271:3967. Torigoe, K.et al. (1997) J. Biol. Chem. 272:25737. Born, T.L.et al. (1998) J. Biol. Chem. 273:29445.

参考图片

IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Human TNF‑ alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL‑18/IL‑1F4 (Catalog # B003-5) stimulates IFN‑ gamma secretion in KG-1 human myeloid leukemia cells in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Human IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑18 R alpha /
IL‑1 R5 Monoclonal Antibody (Catalog # MAB840). The ND₅₀ is typically 0.03‑0.1 µg/mL.

IL‑18 R alpha /IL‑1 R5 in Human PBMCs. IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB840) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.