Human TRANCE/RANK L/TNFSF11 MAb (Clone 70525) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TRANCE/RANK L/TNFSF11 MAb (Clone 70525) (500 UG)

货号： MAB626-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB626-500
别名	CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
全称	TNF-related Activation-induced Cytokine
反应种属	Human
应用	Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human TRANCE/TNFSF11/RANK L in direct ELISAs. No cross-reactivity with recombinant mouse TRANCE is observed.
使用方法	Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in RAW 264.7 mouse macrophages. The Neutralization Dose (ND₅₀) is typically 0.8-2.5 µg/mL in the presence of 30 ng/mL Recombinant Human TRANCE/TNFSF11/RANK L.
来源	Monoclonal Mouse IgG_2B Clone # 70525
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8600 (Human); 21943 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Authors: Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C Bone, 2009;44(6):1143-50. Species: Human Sample Type: Whole Cells Application: Flow alpha1beta1 integrin and interleukin-7 receptor up-regulate the expression of RANKL in human T cells and enhance their osteoclastogenic function. Authors: Gendron S, Boisvert M, Chetoui N, Aoudjit F Immunology, 2008;125(0):359. Species: Human Sample Type: Cell Lysates Application: WB A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and treatment strategies. Authors: Lee N, Smolarz AJ, Olson S, David O, Reiser J, Kutner R, Daw NC, Prockop DJ, Horwitz EM, Gregory CA Br. J. Cancer, 2007;97(11):1552-9. Species: Human Sample Type: Cell Lysates Application: WB Intraarticular corticosteroids decrease synovial RANKL expression in inflammatory arthritis. Authors: Makrygiannakis D, af Klint E, Catrina SB, Botusan IR, Klareskog E, Klareskog L, Ulfgren AK, Catrina AI Arthritis Rheum., 2006;54(5):1463-72. Species: Human Sample Type: Cell Lysates Application: WB Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K. Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT J. Rheumatol., 2005;32(4):713-20. Species: Human Sample Type: Whole Tissue Application: IHC Inflammatory cell infiltrate and RANKL/OPG expression in rheumatoid synovium: comparison with other inflammatory arthropathies and correlation with outcome. Authors: Fonseca JE, Cortez-Dias N, Francisco A, Sobral M, Canhao H, Resende C, Castelao W, Macieira C, Sequeira G, Saraiva F, da Silva JA, Carmo-Fonseca M, Viana Queiroz M Clin. Exp. Rheumatol., 2005;23(2):185-92. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRANCE/TNFSF11/RANK L Gly136-Asp317 (Ala194Gly) Accession # O14788
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2 lacks aa 1-73, and isoform 3 lacks aa 1-47. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart,placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Activated T Cells Mediate Bone Loss Through OPGL/TRANCE Human CD Marker Products ICC / IHC Images: TRANCE/TNFSF11/RANK L Multiple Myeloma Osteoblast and Osteoclast Markers Osteoclast Co-stimulation Regulatory T Cells (Tregs) T Cell Cytokine Signaling TNF Superfamily TNF Superfamily Ligands

参考图片

Osteoclast-Like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in RAW 264.7 mouse macrophages in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) activity in cell lysates. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/
TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626). The ND₅₀ is typically 0.8-2.5 µg/mL.

TRANCE/TNFSF11/RANK L in Human Lymph Node. TRANCE/TNFSF11/RANK L was detected in immersion fixed paraffin-embedded sections of human lymph node using 5 µg/mL Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.