Human Osteoprotegerin/TNFRSF11B MAb (Clone 69146) (500 UG)【科瑞奥博】

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Human Osteoprotegerin/TNFRSF11B MAb (Clone 69146) (500 UG)

货号： MAB805-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB805-500
别名	OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b
反应种属	Human
应用	Neutralization
目标/特异性	Detects human Osteoprotegerin/TNFRSF11B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Osteoprotegerin is observed.
使用方法	Neutralization: Measured by its ability to neutralize Osteoprotegerin/TNFRSF11B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.15-0.3 µg/mL in the presence of 0.1 µg/mL Recombinant Human Osteoprotegerin/TNFRSF11B Fc Chimera, 50 ng/mL Recombinant Human TRAIL/TNFSF10, and 0.5 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 69146
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4982 (Human); 18383 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Selective targeting of death receptor 5 circumvents resistance of MG-63 osteosarcoma cells to TRAIL-induced apoptosis. Authors: Locklin RM, Federici E, Espina B, Hulley PA, Russell RG, Edwards CM Mol. Cancer Ther., 2007;6(12):3219-28. Species: Human Sample Type: Whole Cells Application: Neut Intraarticular corticosteroids decrease synovial RANKL expression in inflammatory arthritis. Authors: Makrygiannakis D, af Klint E, Catrina SB, Botusan IR, Klareskog E, Klareskog L, Ulfgren AK, Catrina AI Arthritis Rheum., 2006;54(5):1463-72. Species: Human Sample Type: Cell Lysates Application: WB Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K. Authors: Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT J. Rheumatol., 2005;32(4):713-20. Species: Human Sample Type: Whole Tissue Application: IHC Inflammatory cell infiltrate and RANKL/OPG expression in rheumatoid synovium: comparison with other inflammatory arthropathies and correlation with outcome. Authors: Fonseca JE, Cortez-Dias N, Francisco A, Sobral M, Canhao H, Resende C, Castelao W, Macieira C, Sequeira G, Saraiva F, da Silva JA, Carmo-Fonseca M, Viana Queiroz M Clin. Exp. Rheumatol., 2005;23(2):185-92. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B Glu22-Leu401 Accession # AAB53709
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Osteoprotegerin is a member of the TNF receptor superfamily expressed by many cell types. It exists in soluble monomeric and dimeric forms as a decoy receptor for its ligands, TRANCE and TRAIL. Osteoprotegerin functions as an antagonist to RANKL-induced osteoclastogenesis and bone resorption.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Gametes and Fertilization Glucose Homeostasis ICC / IHC Images: Osteoprotegerin/TNFRSF11B Regulation of Apoptosis by TNF Superfamily Members TNF Superfamily TNF Superfamily Receptors

参考图片

Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/
TNFSF10-induced Cytotoxicity and Neutralization by Human Osteoprotegerin/
TNFRSF11B Antibody. In the presence of the metabolic inhibitor actinomycin D (0.5 µg/mL), Recombinant Human Osteoprotegerin/TNFRSF11B Fc Chimera (Catalog # 805-OS) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human TRAIL/
TNFSF10 (50 ng/mL) activity elicited by Recombinant Human Osteoprotegerin/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human Osteoprotegerin/TNFRSF11B Monoclonal Antibody (Catalog # MAB805). The ND₅₀ is typically 0.15-0.3 µg/mL.