Mouse G-CSF MAb (Clone 67604) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse G-CSF MAb (Clone 67604) (500 UG)

货号： MAB414-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB414-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB414；Detection antibody：BAF414; and protein: 414-CS-005
别名	C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin
全称	Granulocyte Colony Stimulating Factor
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse G-CSF in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) G‑CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cells line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.
来源	Monoclonal Rat IgG₁ Clone # 67604
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1440 (Human); 12985 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection Authors: Anna A Müller PLoS Pathog, 2016;12(6):e1005723. Species: Mouse Sample Type: In Vivo Application: In vivo Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) Ly6C(+) monocytes that are poised to release TNF-alpha. Authors: Sivakumaran S, Henderson S, Ward S, Santos E Sousa P, Manzo T, Zhang L, Conlan T, Means T, DAveni M, Hermine O, Rubio M, Chakraverty R, Bennett C Eur J Immunol, 2016;46(1):192-203. Species: Mouse Sample Type: In Vivo Application: Neut The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity Cell, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation. Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J Cancer Res, 2015;75(12):2566-79. Species: Mouse Sample Type: Whole Cells Application: Flow IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils. Authors: Trautwein-Weidner K, Gladiator A, Nur S, Diethelm P, LeibundGut-Landmann S Mucosal Immunol, 2015;8(2):221-31. Species: Mouse Sample Type: In Vivo Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi. Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W Stem Cells, 2015;33(3):848-58. Species: Mouse Sample Type: In Vivo Application: Neut G-CSF drives a posttraumatic immune program that protects the host from infection. Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F J Immunol, 2014;192(5):2405-17. Species: Mouse Sample Type: In Vivo Application: In vivo Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism. Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI PLoS ONE, 2011;6(11):e27690. Species: Mouse Sample Type: In Vivo Application: In vivo Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism. Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G PLoS ONE, 2011;6(5):e19957. Species: Mouse Sample Type: In Vivo Application: In vivo Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH J. Clin. Invest., 2011;121(11):4446-61. Species: Mouse Sample Type: In Vivo Application: In vivo Cannabinoid receptor activation leads to massive mobilization of myeloid-derived suppressor cells with potent immunosuppressive properties. Authors: Hegde VL, Nagarkatti M, Nagarkatti PS Eur. J. Immunol., 2010;40(12):3358-71. Species: Mouse Sample Type: In Vivo Application: In vivo G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models. Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7. Species: Mouse Sample Type: In Vivo Application: In vivo The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse. Authors: Furze RC, Rankin SM FASEB J., 2008;22(9):3111-9. Species: Mouse Sample Type: In Vivo Application: In vivo Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow. Authors: Brunner S, Zaruba MM, Huber B, David R, Vallaster M, Assmann G, Mueller-Hoecker J, Franz WM Exp. Hematol., 2008;36(9):1157-66. Species: Mouse Sample Type: In Vivo Application: In vivo The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM Blood, 2007;111(1):42-9. Species: Mouse Sample Type: In Vivo Application: In vivo Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N Nature, 2007;450(7171):825-31. Species: Mouse Sample Type: In Vivo Application: In vivo Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense. Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK J. Exp. Med., 2001;194(4):519-27. Species: Mouse Sample Type: BALF Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse G-CSF Val31-Ala208 Accession # P09920
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the G_o period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.
运输条件	Blue Ice
存放说明	-20℃
参考文献	A novel role for the nervous system in HSPS mobilization ALS Related Molecules Cytokines and Receptors in Angiogenesis Gametes and Fertilization IL-6 Family Macrophage Activation Markers

参考图片

Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody.

Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the NFS‑60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G‑CSF Monoclonal Antibody (Catalog # MAB414). The ND₅₀ is typically 0.01-0.03 µg/mL.