Human CCL20/MIP-3 alpha MAb (Clone 67310) (500 UG)【科瑞奥博】

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Human CCL20/MIP-3 alpha MAb (Clone 67310) (500 UG)

货号： MAB360-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB360-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB360；Detection antibody：BAF360; and protein: 360-MP-025
别名	beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38
反应种属	Human
应用	Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CCL20/MIP-3 alpha in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CCL19, rhCXCL1, rhCCL16, rhCCL11, recombinant mouse CCL20, or recombinant rat CCL20.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Human CCL20/MIP‑3 alpha.
来源	Monoclonal Mouse IgG₁ Clone # 67310
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6364 (Human); 20297 (Mouse); 29538 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination. Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z Autophagy, 2014;10(2):257-68. Species: Human Sample Type: Whole Cells Application: Neut IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma. Authors: Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S Clin. Cancer Res., 2011;17(24):7529-38. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Expression of the Epstein-Barr virus-encoded Epstein-Barr virus nuclear antigen 1 in Hodgkin's lymphoma cells mediates Up-regulation of CCL20 and the migration of regulatory T cells. Authors: Baumforth KR, Birgersdotter A, Reynolds GM, Wei W, Kapatai G, Flavell JR, Kalk E, Piper K, Lee S, Machado L, Hadley K, Sundblad A, Sjoberg J, Bjorkholm M, Porwit AA, Yap LF, Teo S, Grundy RG, Young LS, Ernberg I, Woodman CB, Murray PG Am. J. Pathol., 2008;173(1):195-204. Species: Human Sample Type: Whole Cells Application: Bioassay Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG J. Immunol., 2008;181(10):7420-7. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua. Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ J. Pathol., 2008;214(3):328-36. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C Blood, 2007;109(9):3649-57. Species: Human Sample Type: Plasma Application: ELISA Development Substance P regulates macrophage inflammatory protein 3alpha/chemokine C-C ligand 20 (CCL20) with heme oxygenase-1 in human periodontal ligament cells. Authors: Lee SK, Pi SH, Kim SH, Min KS, Lee HJ, Chang HS, Kang KH, Kim HR, Shin HI, Lee SK, Kim EC Clin. Exp. Immunol., 2007;150(3):567-75. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Th2 cytokine response in Major Depressive Disorder patients before treatment. Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G J. Neuroimmunol., 2006;172(1):156-65. Species: Human Sample Type: Serum Application: ELISA Development Hypoxia modifies the transcriptome of primary human monocytes: modulation of novel immune-related genes and identification of CC-chemokine ligand 20 as a new hypoxia-inducible gene. Authors: Bosco MC, Puppo M, Santangelo C, Anfosso L, Pfeffer U, Fardin P, Battaglia F, Varesio L J. Immunol., 2006;177(3):1941-55. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL20/MIP-3 alpha Ala27-Met96 (Asn95Asp) Accession # P78556.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17. CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Baba, M. et al. (1997) J. Biol. Chem. 272:14893. Hromas, R. et al. (1997) Blood 89:3315. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.

参考图片

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Human CCL20/MIP‑3 alpha Antibody. Recombinant Human CCL20/MIP‑3 alpha (Catalog # 360-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP‑3 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB360). The ND₅₀ is typically 1-5 µg/mL.