Human IL-6 MAb (Clone 6708) (500 UG)【科瑞奥博】

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Human IL-6 MAb (Clone 6708) (500 UG)

货号： MAB206-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB206-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB206；Detection antibody：BAF206; and protein: 206-IL-010
别名	B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta 2; IFN-beta-2; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
全称	Interleukin 6
反应种属	Human
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human and primate IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL‑6, rhOSM, rhLIF, rhIL‑11, rhgp130, or rhCNTF.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND₅₀) is typically 0.05-0.15 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.
来源	Monoclonal Mouse IgG₁ Clone # 6708
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium PLoS ONE, 2016;11(5):e0155161. Species: Human Sample Type: Whole Cells Application: Neut CXCL8 and CCL20 Enhance Osteoclastogenesis via Modulation of Cytokine Production by Human Primary Osteoblasts. Authors: Pathak J, Bakker A, Verschueren P, Lems W, Luyten F, Klein-Nulend J, Bravenboer N PLoS ONE, 2015;10(6):e0131041. Species: Human Sample Type: Whole Cells Application: Neut Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition. Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J Endocrinology, 2015;156(2):670-9. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Capture Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis. Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T J Biol Chem, 2015;290(36):22212-24. Species: Mouse Sample Type: In Vivo Application: Neut Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner. Authors: Mace T, Ameen Z, Collins A, Wojcik S, Mair M, Young G, Fuchs J, Eubank T, Frankel W, Bekaii-Saab T, Bloomston M, Lesinski G Cancer Res, 2014;73(10):3007-18. Species: Human Sample Type: Whole Cells Application: Neut Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk. Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C J Immunol, 2014;193(1):234-43. Species: Human Sample Type: Whole Cells Application: Neut Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays. Authors: Todd J, Simpson P, Estis J, Torres V, Wub A Cytokine, 2013;64(3):660-5. Species: Human Sample Type: Plasma Application: ELISA Development Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. Authors: Nicolaidou V, Wong MM, Redpath AN PLoS ONE, 2012;7(7):e39871. Species: Human Sample Type: Whole Cells Application: Neut Protection from RNA and DNA viruses by IL-32. Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF J. Immunol., 2011;186(7):4110-8. Species: Human Sample Type: Cell Culture Supernates Application: Electrochemiluminescence Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Authors: Tso GH, Law HK, Tu W Stem Cells, 2010;28(5):939-54. Species: Human Sample Type: Whole Cells Application: Neut Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23. Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M Eur. J. Immunol., 2009;39(5):1221-30. Species: Human Sample Type: Whole Cells Application: Neut Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome. Authors: Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD J. Immunol., 2008;180(11):7368-75. Species: Human Sample Type: Whole Cells Application: Neut Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6. Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P J. Immunol., 2008;181(4):2772-80. Species: Human Sample Type: Whole Cells Application: Neut Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Authors: Osei-Kumah A, Smith R, Clifton VL Cytokine, 2008;43(2):187-93. Species: Human Sample Type: Plasma Application: ELISA Development Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines. Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE AIDS Res. Hum. Retroviruses, 2008;24(5):667-71. Species: Human Sample Type: Vaginal Fluid Application: ELISA Development Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation. Authors: Kurago ZB, Lam-ubol A, Stetsenko A Head Neck Pathol, 2008;2(1):1-12. Species: Human Sample Type: Whole Cells Application: Neut Endogenous IL-32 controls cytokine and HIV-1 production. Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA J. Immunol., 2008;181(1):557-65. Species: Human Sample Type: Cell Culture Supernates Application: Electrochemiluminescence Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. Authors: Arnold R, Neumann M, Konig W Immunology, 2007;121(1):71-81. Species: Human Sample Type: Whole Cells Application: Neut Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose. Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M J. Clin. Oncol., 2007;25(29):4542-9. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F Stem Cells, 2007;26(1):279-89. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Th2 cytokine response in Major Depressive Disorder patients before treatment. Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G J. Neuroimmunol., 2006;172(1):156-65. Species: Human Sample Type: Serum Application: ELISA Development Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Arthritis Rheum., 2006;54(7):2096-108. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery. Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B Acta Obstet Gynecol Scand, 2005;84(6):551-7. Species: Human Sample Type: Amniotic Fluid Application: ELISA Development Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. Authors: Copeland S, Siddiqui J, Remick D J. Immunol. Methods, 2004;284(1):99-106. Species: Human Sample Type: Cell Culture Supernates Application: Array Development Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Authors: Phillips TM Electrophoresis, 2004;25(10):1652-9. Species: Human Sample Type: Serum Application: Affinity Chromatography trans fatty acids and systemic inflammation in heart failure. Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC Am. J. Clin. Nutr., 2004;80(6):1521-5. Species: Human Sample Type: Plasma Application: ELISA Development Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation. Authors: McLoughlin RM, Witowski J, Robson RL, Wilkinson TS, Hurst SM, Williams AS, Williams JD, Rose-John S, Jones SA, Topley N J. Clin. Invest., 2003;112(4):598-607. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Peritoneal Fluid Application: ELISA Development The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes. Authors: Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A Arthritis Rheum., 2001;44(10):2392-401. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK J Korean Med Sci, 0;26(9):1132-9. Species: Human Sample Type: Serum Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL-6
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Van Snick, J. (1990) Annu. Rev. Immunol. 8:253. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502. Jones, S.A. (2005) J. Immunol. 175:3468. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227. Hirano, T. et al. (1986) Nature 324:73. Alberti, L. et al. (2005) Cancer Res. 65:2. Kestler, D.P. et al. (1995) Blood 86:4559. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.

参考图片

IL‑6 in Human Skin. IL‑6 was detected in immersion fixed frozen sections of hyperplastic human skin using Mouse Anti-Human/Primate IL‑6 Monoclonal Antibody (Catalog # MAB206) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate
IL‑6 Monoclonal Antibody (Catalog # MAB206). The ND₅₀ is typically 0.05-0.15 µg/mL.