Human BMP-4 MAb (Clone 66119) (500 UG)【科瑞奥博】

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Human BMP-4 MAb (Clone 66119) (500 UG)

货号： MAB757-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB757-500
别名	BMP-2B; BMP2B1; BMP2BMCOPS6; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME
全称	Bone Morphogenetic Protein 4
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the C2C12 mouse myoblast cell line. Katagiri, T. et al. (1994) J. Cell Biol. 127:1755. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 500 ng/mL Recombinant Human BMP‑4 and 2 µg/mL heparin.
来源	Monoclonal Mouse IgG_2B Clone # 66119
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. Authors: Herrera B, Inman GJ, BMC Cell Biol., 2009;10(0):20. Species: Human Sample Type: recombinant BMP-4 Application: Neut Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis. Authors: Kane R, Godson C, OBrien C Mol. Vis., 2008;14(0):1138-48. Species: Human Sample Type: Cell Lysates Application: WB BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V Blood, 2008;112(8):3154-63. Species: Human Sample Type: Whole Cells Application: Neut Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions. Authors: Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH Ann. Neurol., 2007;62(3):288-300. Species: Rat Sample Type: Whole Tissue Application: IHC Frozen Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells. Authors: Zode GS, Clark AF, Wordinger RJ Invest. Ophthalmol. Vis. Sci., 2007;48(11):5058-67. Species: Human Sample Type: Tissue Homogenates Application: WB Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach. Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR J. Pathol., 2006;209(2):190-7. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation. Authors: Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C Mol. Cell. Biol., 2003;23(16):5664-79. Species: Human Sample Type: recombinant BMP-4 Application: Neut Sonic hedgehog regulates gastric gland morphogenesis in man and mouse. Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP Gastroenterology, 2001;121(2):317-28. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human BMP‑4 Ser293-Arg408 Accession # P12644
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Zhang, P. et al., 2008, Blood 111:1933. Gambaro, K. et al., 2006, Cell Death Differ. 13:1075. Simic, P. and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299. Sadlon, T.J. et al., 2004, Stem Cells 22:457. Frank, D.B. et al., 2005, Circ. Res. 97:496. Wozney, J. et al., 1988, Science 242:1528. Cui, Y. et al., 1998, EMBO J. 17:4735. Cui, Y. et al., 2001, Genes Dev. 15:2797. Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670. Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75. Chen, D. et al., 2004, Growth Factors 22:233. Lavery, K. et al., 2008, J. Biol. Chem. 283:20948. Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19. Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12. Takada, T. et al., 2003, J. Biol. Chem. 278:43229.

参考图片

Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND₅₀ is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).