| 货号 | MAB465-500 |
| 别名 | PlGF2; PlGF-2 | 全称 | Placenta Growth Factor 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse PlGF-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PlGF or rhVEGF is observed. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.015 - 0.06 μg/mL of this antibody will block 50% of the binding of 4 ng/mL of recombinant mouse PlGF-2 to immobilized recombinant human Flt-1/Fc Chimera coated at 1 μg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Rat IgG2A Clone # 62526 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5228 (Human); 18654 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Temporal expression of growth factors triggered by epiregulin regulates inflammation development. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2 Ala24-Pro158 Accession # P49764 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | PlGF is a member of the VEGF family of growth factors, and it binds with high-affinity to VEGF R1. As a result of alternative splicing, three human PlGF mRNAs encode PlGF-1 (PlGF131), PlGF-2 (PlGF152) and PlGF-3 (PlGF203). Normal mouse tissues express only a 158 amino acid precursor that is the equivalent of human PlGF-2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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