Mouse CCL9/10/MIP-1 gamma MAb (Clone 62105) (500 UG)【科瑞奥博】

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Mouse CCL9/10/MIP-1 gamma MAb (Clone 62105) (500 UG)

货号： MAB463-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB463-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB463-500；Detection antibody：BAF463; and protein: 463-MG-025
别名	CCF18; MIP-1 gamma
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CCL9/10/MIP‑1 gamma in ELISAs and Western blots. In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human CCL3, 4, 15, 19, 20, recombinant mouse CCL3, 4, 19, or recombinant rat CCL20 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL9/10/MIP‑1 gamma -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL9/10/MIP‑1 gamma.
来源	Monoclonal Rat IgG₁ Clone # 62105
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	20308 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions. Authors: Mosmann T Eur. J. Immunol., 2004;34(6):1617-26. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival. Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P J. Immunol., 2004;173(3):2084-90. Species: Mouse Sample Type: Whole Cells Application: Neut MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival. Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P J. Immunol., 2004;173(3):2084-90. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CCL9/10/MIP‑1 gamma Gln22-Gln122 Accession # P51670
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full‑length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Zlotnik, A. and O. Yoshie (2000) Immunity 12:121. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1 Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102. Haelens, A. et al. (1996) Immunobiology 195:499. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341. Youn, B-S. et al. (1995) J. Immunol. 155:2661. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.

参考图片

Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutralization by Mouse CCL9/10/
MIP‑1 gamma Antibody.
Recombinant Mouse CCL9/10/
MIP‑1 gamma (Catalog # 463-MG) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP‑1 gamma (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL9/10/MIP‑1 gamma Monoclonal Antibody (Catalog # MAB463). The ND₅₀ is typically 1-4 µg/mL.