Human uPAR MAb (Clone 62022) (500 UG)【科瑞奥博】

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Human uPAR MAb (Clone 62022) (500 UG)

货号： MAB807-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB807-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB807；Detection antibody：BAF807; and protein: 807-UK-100
别名	CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3
全称	Urokinase-type Plasminogen Activator Receptor
反应种属	Human
应用	Western Blot,Flow Cytometry,Blockade of Receptor-ligand Interaction,CyTOF-ready,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard
目标/特异性	Detects human uPAR in direct ELISAs and Western blots. When used in a sandwich ELISA in combination with the biotinylated anti-human uPAR detection antibody (Catalog # BAF807), no significant cross-reactivity was observed with recombinant mouse uPAR.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.5-1.5 µg/mL of this antibody will block 50% of the binding of 30 ng/mL of Recombinant Human uPAR (Catalog # 807-UK) to immobilized Recombinant Human u-Plasminogen Activator/Urokinase (Catalog # 1310-SE) coated at 500 ng/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard :
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5329 (Human); 18793 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The periodontal pathogen Porphyromonas gingivalis preferentially interacts with oral epithelial cells in S phase of the cell cycle Authors: FB Al-Taweel, CW Douglas, SA Whawell Infect Immun, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Soluble Urokinase Receptor Is Released Selectively by Glioblastoma Cells That Express Epidermal Growth Factor Receptor Variant III and Promotes Tumor Cell Migration and Invasion. Authors: Gilder A, Jones K, Hu J, Wang L, Chen C, Carter B, Gonias S J Biol Chem, 2015;290(24):14798-809. Species: Human Sample Type: Whole Cells Application: Neut Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines. Authors: Kacsinta A, Rubenstein C, Sroka I, Pawar S, Gard J, Nagle R, Cress A Biochem Biophys Res Commun, 2014;454(2):335-40. Species: Human Sample Type: Cell Lysate Application: WB Plasmin cleavage of von Willebrand factor as an emergency bypass for ADAMTS13 deficiency in thrombotic microangiopathy. Authors: Tersteeg C, de Maat S, De Meyer S, Smeets M, Barendrecht A, Roest M, Pasterkamp G, Fijnheer R, Vanhoorelbeke K, de Groot P, Maas C Circulation, 2014;129(12):1320-31. Species: Human Sample Type: Whole Cells Application: Functional Assay Activation of the plasma kallikrein-kinin system on human lung epithelial cells. Authors: Vergiliana JF, Asokananthan N, Stewart GA Biol. Chem., 2010;391(9):1067-77. Species: Human Sample Type: Whole Cells Application: Flow Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development AP-1-controlled hepatocyte growth factor activation promotes keratinocyte migration via CEACAM1 and urokinase plasminogen activator/urokinase plasminogen receptor. Authors: Schnickmann S, Camacho-Trullio D, Bissinger M, Eils R, Angel P, Schirmacher P, Szabowski A, Breuhahn K J. Invest. Dermatol., 2009;129(5):1140-8. Species: Human Sample Type: Cell Lysates Application: WB Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma. Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB Am. J. Respir. Cell Mol. Biol., 2006;35(4):503-11. Species: Human Sample Type: Whole Cells Application: Neut LDL-receptor-related protein regulates beta2-integrin-mediated leukocyte adhesion. Authors: Spijkers PP, da Costa Martins P, Westein E, Gahmberg CG, Zwaginga JJ, Lenting PJ Blood, 2005;105(1):170-7. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	Mouse myeloma cell line NS0-derived recombinant human uPAR Leu23-Arg303 Accession # Q03405
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin, a serine protease that is involved in a variety of normal and pathological processes that require cell migration and/or tissue destruction. uPA is synthesized and released from cells as a single-chain (sc) pro-enzyme with limited enzymatic activity and is converted to an active two-chain (tc) disulfide-linked active enzyme by plasmin and other specific proteinases. Both the scuPA and tcuPA bind with high-affinity to the cell surface via the glycosyl phosphatidylinositol-linked receptor uPAR which serves to localize the uPA proteolytic activity. The enzymatic activity of scuPA has also been shown to be enhanced by binding to uPAR. Independent of their proteolytic activity, the uPA/uPAR interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site on uPAR. uPAR cDNA encodes a 335 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, five potential N-linked glycosylation sites and a C‑terminal GPI-anchor site. An alternate spliced variant of uPAR encoding a secreted soluble form of uPAR also exists. Human and mouse uPAR share approximately 60% aa sequence identity and the receptor-ligand interaction is strictly species-specific.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Dear, A.E. and R.L. Medcalf (1988) Eur. J. Biochemistry 252:185.

参考图片

Detection of uPAR in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human uPAR Monoclonal Antibody (Catalog # MAB807, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.