Human M-CSF R MAb (Clone 61701) (500 UG)【科瑞奥博】

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Human M-CSF R MAb (Clone 61701) (500 UG)

货号： MAB3291-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB3291-500
别名	CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; Proto-oncogene c-Fms
全称	Macrophage Colony Stimulating Factor Receptor
反应种属	Human
应用	Western Blot(2 µg/mL)
目标/特异性	Detects human M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
使用方法	Western Blot: 2 µg/mL Neutralization: Measured by its ability to neutralize M-CSF R/CD115-mediated inhibition of proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. The Neutralization Dose (ND₅₀) is typically 4-16 ng/mL in the presence of 30 ng/mL Recombinant Human M-CSF R/CD115 Fc Chimera and 1 ng/mL Recombinant Human M-CSF.
来源	Monoclonal Mouse IgG₁ Clone # 61701
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1436 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFbeta in claudin-low breast tumor cells. Authors: Patsialou A, Wang Y, Pignatelli J, Chen X, Entenberg D, Oktay M, Condeelis J Oncogene, 2015;34(21):2721-31. Species: Human Sample Type: Whole Cells Application: Neut Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma. Authors: Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S Nat. Med., 2010;16(5):571-9. Species: Human Sample Type: Cell Lysates Application: IP
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115 Ile20-Glu512 (Pro54Ala) Accession # P07333.2
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	M-CSF receptor, the product of the c-fmsproto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha _vbeta₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).
运输条件	Blue Ice
存放说明	-20℃
参考文献	deParseval, N. et al. (1993) Nucleic Acids Res. 21:750. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88. Rovida, E. et al. (2001) J. Immunol. 166:1583. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128. Dai, X. et al. (2002) Blood 99:111. Faccio, R. et al. (2003) J. Clin. Invest. 111:749. Li, M. et al. (2004) J. Neurochem. 91:623. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

参考图片

Detection of Human M-CSF R/CD115 by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for M-CSF R/CD115 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

M-CSF R/CD115 Inhibition of M-CSF-dependent Cell Proliferation and Neutralization by Human
M-CSF R/CD115 Antibody. Recombinant Human
M-CSF R/CD115 Fc Chimera (Catalog # 329-MR) inhibits Recombinant Human M-CSF (Catalog # 216-MC) induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human M-CSF R/CD115 (1 ng/mL) activity elicited by Recombinant Human
M-CSF R/CD115 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human M-CSF R/CD115 Monoclonal Antibody (Catalog # MAB3291). The ND₅₀ is typically 4-16 ng/mL.