Mouse TNF RI/TNFRSF1A MAb (Clone 55R170) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse TNF RI/TNFRSF1A MAb (Clone 55R170) (500 UG)

货号： MAB430-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB430-500
别名	CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
全称	Tumor Necrosis Factor Receptor I
反应种属	Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD27, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rmEDAR, rhFas, rmFas, rhEDAR, rhGITR, rmGITR, rhHVEM, rhLT beta R, rmLT beta R, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rhTNF RII or rmTNF RII is observed.
使用方法	Western Blot: 1 µg/mL Immunoprecipitation: Sheehan, K. et al. (1995) J. Exp. Med. 181:607. Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 5-20 µg/mL in the presence of 0.1 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Monoclonal Hamster IgG Clone # 55R170
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Authors: Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V Nature, 2015;528(7582):370-5. Species: Mouse Sample Type: Cell Lysates Application: WB Lipopolysaccharide triggered TNF-alpha-induced hepatocyte apoptosis in a murine non-alcoholic steatohepatitis model. Authors: Kudo H, Takahara T, Yata Y, Kawai K, Zhang W, Sugiyama T J. Hepatol., 2009;51(1):168-75. Species: Mouse Sample Type: In Vivo Application: In vivo Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages. Authors: Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H Am. J. Physiol., Cell Physiol., 2008;295(1):C2-12. Species: Mouse Sample Type: Cell Lysates Application: IP TLR-2 is upregulated and mobilized to the hepatocyte plasma membrane in the space of Disse and to the Kupffer cells TLR-4 dependently during acute endotoxemia in mice. Authors: Ojaniemi M, Liljeroos M, Harju K, Sormunen R, Vuolteenaho R, Hallman M Immunol. Lett., 2005;102(2):158-68. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Recombinant mouse TNF RI/TNFRSF1A Extracellular domain
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1‑3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6‑8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa ECD with a PLAD domain (5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, porcine, human, and rat TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Peschon, J.J. et al. (1998) J. Immunol. 160:943. Banner, D.W et al. (1993) Cell 73: 431. Chan, F.K. et al. (2000) Science 288:2351. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367. Jin, L. et al. (2000) J. Immunol. 165:5153. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190. Legler, D.F. et al. (2003) Immunity 18:655. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969. Islam, A. et al. (2006) J. Biol. Chem. 281:6860. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105. McDermott, M.F. et al. (1999) Cell 97:133. Schneider-Brachert, W. et al. (2004) Immunity 21:415. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

参考图片

Cytotoxicity Induced by
TNF‑ alpha and Neutralization by Mouse TNF RI/TNFRSF1A Antibody. Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB430). The ND₅₀ is typically 5-20 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).