Human CCL17/TARC MAb (Clone 54026) (500 UG)【科瑞奥博】

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Human CCL17/TARC MAb (Clone 54026) (500 UG)

货号： MAB364-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB364-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB364；Detection antibody：BAF364; and protein: 364-DN-025
别名	ABCD-2; CC chemokine TARC; C-C motif chemokine 17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human CCL17/TARC in ELISAs. Does not cross-react with recombinant human(rh) CCL2, 3, 4, 5, 7, 8, 11, 13, 16, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL2, 3,4, 5, 7, 9/10/MIP-1 gamma, 11, 12, 21, 22, 25, or rrCCL20.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 10 ng/mL Recombinant Human CCL17/TARC.
来源	Monoclonal Mouse IgG₁ Clone # 54026
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6361 (Human); 20295 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Lung transplantation affects expression of the chemokine receptor type 4 on specific T cell subsets. Authors: Paantjens AW, van de Graaf EA, Kwakkel-van Erp JM, Hoefnagel T, van Kessel DA, van den Bosch JM, Otten HG Clin. Exp. Immunol., 2011;166(1):103-9. Species: Human Sample Type: Serum Application: ELISA Development CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer. Authors: Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H Int. J. Cancer, 2008;122(10):2286-93. Species: Human Sample Type: Whole Cells Application: Bioassay Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA J. Immunol., 2007;179(7):4840-8. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis. Authors: Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B Blood, 2007;111(2):761-6. Species: Human Sample Type: Whole Cells Application: Neut Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping Nat Med, 2004;10(9):942-9. Species: Human Sample Type: Whole Cells Application: Neut Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Authors: Liu L, Jarjour NN, Busse WW, Kelly EA Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24. Species: Human Sample Type: BALF Application: ELISA Development TARC augments TNF-alpha-induced CTACK production in keratinocytes. Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M Exp. Dermatol., 2004;13(9):551-7. Species: Human Sample Type: Cell Culture Supernates Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios. Authors: Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN J. Immunol., 2001;166(11):6899-906. Species: Human Sample Type: Synovial Fluid Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL17/TARC Ala24-Ser94 Accession # Q92583.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Imai, T. et al. (1997) J. Biol. Chem. 272:15036. Imai, T.et al. (1996) J. Biol. Chem. 271:21514. Nomiyama, H. et al. (1997) Genomics 40:211.

参考图片

Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/TARC (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL17/TARC Monoclonal Antibody (Catalog # MAB364). The ND₅₀ is typically 0.4-2.0 µg/mL.