Human IL-2 MAb (Clone 5334) (500 UG)【科瑞奥博】

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Human IL-2 MAb (Clone 5334) (500 UG)

货号： MAB202-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB202-500
别名	aldesleukin; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
全称	Interleukin 2
反应种属	Human
应用	Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
使用方法	Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.015-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑2.
来源	Monoclonal Mouse IgG₁ Clone # 5334
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activin A programs the differentiation of human TFH cells Nat Immunol, 2016;17(8):976-84. Species: Human Sample Type: Whole Cells Application: Bioassay Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y J Biol Chem, 2015;290(37):22338-51. Species: Human Sample Type: Proteins Application: Neut ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 2012;119(26):6268-77. Species: Human Sample Type: Whole Cells Application: Neut Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive. Authors: Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR Vaccine, 2010;28(8):1932-41. Species: Human Sample Type: Whole Cells Application: Flow T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue. Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC Blood, 2007;109(11):4856-64. Species: Human Sample Type: Whole Cells Application: Neut Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue. Authors: Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, KytÃƒÂ¶lÃƒÂ¤ S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A Cancer Res., 2005;65(18):8101-10. Species: Human Sample Type: Whole Cells Application: Flow Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Immunology, 2004;112(2):274-89. Species: Human Sample Type: Whole Cells Application: Flow Reprogramming of IL-10 activity and signaling by IFN-gamma. Authors: Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB J. Immunol., 2003;171(10):5034-41. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL‑2 Ala21-Thr153 Accession # NP_000577
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.
运输条件	Blue Ice
存放说明	-20℃
参考文献	BIObrief: Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions Cancer Biomarkers Common gamma Chain Receptor Family Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells Group 1 ILCs ICC / IHC Images: IL-2 Infectious Diseases Inflammatory Mediators Interactive Pathway: IL-2 Signaling and its Primary Biological Effects in Different Immune Cell Types Interactive Pathway: IL-2 Signaling Pathways Isolation, Culture, and Identification of Natural Killer Cells Mini-review: Cytolytic Granule-mediated Apoptosis Multiple Sclerosis and Myelin Related Molecules NCR+ILC3 Neuroinflammation Preeclampsia Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells Th9 Cells

参考图片

Cell Proliferation Induced by IL‑2 and Neutralization by Human IL‑2 Antibody. Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202). The ND₅₀ is typically 0.015-0.03 µg/mL.

IL‑2 in Human PBMCs.
IL‑2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.