Human CXCL9/MIG MAb (Clone 49106) (500 UG)【科瑞奥博】

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Human CXCL9/MIG MAb (Clone 49106) (500 UG)

货号： MAB392-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB392-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB392；Detection antibody：BAF392; and protein: 392-MG-010
别名	chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma
反应种属	Human
应用	Western Blot,CyTOF-ready,Immunocytochemistry,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9, recombinant human CXCL10.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND₅₀) is typically 4-20 µg/mL in the presence of 0.25 µg/mL Recombinant Human CXCL9/MIG.
来源	Monoclonal Mouse IgG₁ Clone # 49106
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4283 (Human); 17329 (Mouse); 246759 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCL9 and CXCL10 predict survival and are regulated by cyclooxygenase inhibition in advanced serous ovarian cancer Br J Cancer, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: ICC Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus. Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J J. Invest. Dermatol., 2011;131(1):133-40. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH J. Immunol., 2010;184(6):2886-98. Species: Human Sample Type: Whole Cells Application: Neut Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: ICC CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis. Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA Eur. Respir. J., 2009;34(3):676-86. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D J. Immunol., 2007;179(6):4272-82. Species: Human Sample Type: Serum Application: Immunodepletion HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma. Authors: Suyama T, Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ichikawa T, Ueda T, Nikaido T, Ito H, Ishikura H Cancer, 2005;103(2):258-67. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow. Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH Am. J. Pathol., 2005;167(3):887-99. Species: Human Sample Type: Tissue Homogenates Application: WB Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D J. Immunol., 2004;173(11):6592-602. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human CXCL9/MIG Thr23-Thr125 Accession # Q07325
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL9, a member of the alpha subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9was subsequently cloned using mouse MIG cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide that is cleaved to yield a 103 aa residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic aa residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes. The E. coli-expressed CXCL9 preparations produced at R&D Systems have been shown to contain greater than 80% full length CXCL9.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Loetscher, M. et al. (1996) J. Exp. Med. 184:963. Liao, F. et al. (1995) J. Exp. Med. 182:1301. Vanguri, P. (1995) J. Neuroimmunol. 56:35.

参考图片

CXCL9/MIG in THP-1 Human Cell Line. CXCL9/MIG was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line stimulated with IFN-gamma for 24 hours using Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody. Recombinant Human CXCL9/MIG (Catalog # 392-MG) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL9/MIG (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392). The ND₅₀ is typically 4-20 µg/mL.