Mouse CXCL1/KC MAb (Clone 48415) (500 UG)【科瑞奥博】

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Mouse CXCL1/KC MAb (Clone 48415) (500 UG)

货号： MAB453-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB453-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB453；Detection antibody：BAF453; and protein: 453-KC-010
别名	CINC-1; GRO alpha; KC; MGSA-alpha
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs and Western blots. Shows 100% cross‑reactivity with recombinant human (rh) CXCL2, less than 40% cross‑reactivity with recombinant rat (rr) CINC-1, and no cross‑reactivity with rrCINC-3, rhCXCL3, rhCXCL5, rhCXCL6, or recombinant mouse CXCL6.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96.
来源	Monoclonal Rat IgG_2A Clone # 48415
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2919 (Human); 14825 (Mouse); 81503 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype PLoS ONE, 2016;11(8):e0159986. Species: Mouse Sample Type: Whole Cells Application: Bioassay The human amniotic fluid stem cell secretome effectively counteracts doxorubicin-induced cardiotoxicity Sci Rep, 2016;6(0):29994. Species: Mouse Sample Type: Whole Cells Application: Neut The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression Authors: L Seifert, G Werba, S Tiwari, NN Giao Ly, S Alothman, D Alqunaibit, A Avanzi, R Barilla, D Daley, SH Greco, A Torres-Her, M Pergamo, A Ochi, CP Zambirinis, M Pansari, M Rendon, D Tippens, M Hundeyin, VR Mani, C Hajdu, D Engle, G Miller Nature, 2016;532(7598):245-9. Species: Mouse Sample Type: In Vivo Application: In vivo Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo MicroRNA-146a provides feedback regulation of lyme arthritis but not carditis during infection with Borrelia burgdorferi. Authors: Lochhead R, Ma Y, Zachary J, Baltimore D, Zhao J, Weis J, OConnell R, Weis J PLoS Pathog, 2014;10(6):e1004212. Species: Mouse Sample Type: Serum Application: ELISA Development CXCL1 but not IL-6 is required for recurrent herpetic stromal keratitis. Authors: West D, Del Rosso C, Yin X, Stuart P J Immunol, 2014;192(4):1762-7. Species: Mouse Sample Type: In Vivo Application: Neut Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. Authors: Roy M, Richard JF, Dumas A, Vallieres L J Neuroinflammation, 2012;9(0):18. Species: Mouse Sample Type: In Vivo Application: In vivo Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH J. Clin. Invest., 2011;121(11):4446-61. Species: Mouse Sample Type: In Vivo Application: In vivo Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Am. J. Pathol., 2011;179(6):2730-9. Species: Mouse Sample Type: BALF Application: ELISA Development Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis. Authors: Summers SA, van der Veen BS, OSullivan KM Kidney Int., 2010;78(12):1263-74. Species: Mouse Sample Type: In Vivo Application: In vivo Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis. Authors: Chou RC, Kim ND, Sadik CD Immunity, 2010;33(2):266-78. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Interleukin 17 sustains rather than induces inflammation. Authors: Maione F, Paschalidis N, Mascolo N, Dufton N, Perretti M, DAcquisto F Biochem. Pharmacol., 2009;77(5):878-87. Species: Mouse Sample Type: In Vivo Application: In vivo Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T Microbes Infect., 2009;11(4):524-30. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T Immunology, 2008;125(2):272-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways. Authors: De Filippo K, Henderson RB, Laschinger M, Hogg N J. Immunol., 2008;180(6):4308-15. Species: Mouse Sample Type: In Vivo Application: In vivo HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling. Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM Blood, 2008;111(7):3343-54. Species: Mouse Sample Type: In Vivo Application: In vivo Keratocan and lumican regulate neutrophil infiltration and corneal clarity in lipopolysaccharide-induced keratitis by direct interaction with CXCL1. Authors: Carlson EC, Lin M, Liu CY, Kao WW, Perez VL, Pearlman E J. Biol. Chem., 2007;282(49):35502-9. Species: Mouse Sample Type: Tissue Homogenates Application: IP The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM Blood, 2007;111(1):42-9. Species: Mouse Sample Type: In Vivo Application: In vivo TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor. Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ Eur. J. Pharmacol., 2007;573(1):221-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA J. Immunol., 2006;177(1):612-20. Species: Mouse Sample Type: BALF Application: ELISA Development Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A J. Immunol., 2006;177(12):8650-7. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Infect. Immun., 2006;74(12):6730-8. Species: Mouse Sample Type: BALF Application: ELISA Development Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice. Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG Inflamm. Res., 2006;55(11):457-64. Species: Mouse Sample Type: Synovial Fluid Application: ELISA Development Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Eur. J. Immunol., 2006;36(8):2025-34. Species: Mouse Sample Type: Peritoneal Exudate Application: ELISA Development Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation. Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD J. Immunol., 2005;175(4):2589-97. Species: Mouse Sample Type: Tissue Homogenates Application: WB Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Selective macrophage suppression during sepsis. Authors: Ellaban E, Bolgos G, Remick D Cell. Immunol., 2004;231(1):103-11. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Regulatory effects of eotaxin on acute lung inflammatory injury. Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA J. Immunol., 2001;166(8):5208-18. Species: Mouse Sample Type: BALF Application: ELISA Development Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation. Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP J. Immunol., 2001;166(2):1233-40. Species: Mouse Sample Type: In Vivo Application: In vivo Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Immunity, 0;43(5):945-58. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Capture
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1 Arg20-Lys96 Accession # P12850
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL1, also known as KC and GRO alpha, is a CXC family chemokine that is expressed in fibroblasts, macrophages, and endothelial cells and interacts with CXCR2. CXCL1 is a potent neutrophil attractant and activator. It also promotes angiogenesis by inducing VEGF production by neutrophils. N-terminally truncated CXCL1 demonstrates increased potency in CXCR2 signaling. Mature mouse CXCL1 shares 96% and 64% aa sequence identity with rat and human CXCL1, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors ICC / IHC Images: CXCL1/GRO alpha/KC/CINC-1 Inflammatory Mediators Macrophage Activation Markers

参考图片

Chemotaxis Induced by CXCL1/GRO alpha /KC/CINC‑1 and Neutralization by Mouse CXCL1/GRO alpha /KC/CINC‑1 Antibody. Recombinant Mouse CXCL1/GRO alpha /KC/
CINC‑1 aa 20‑96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20‑96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453). The ND₅₀ is typically
0.05‑0.25 µg/mL.