Rat TNF-alpha MAb (Clone 45418) (500 UG)【科瑞奥博】

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Rat TNF-alpha MAb (Clone 45418) (500 UG)

货号： MAB510-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB510-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB510-500 or MAB510R-100；Detection antibody：BAF510; and protein: 510-RT-010
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Rat
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects rat TNF-alpha in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF‑ alpha and less than 0.2% cross-reactivity with rhTNF‑ alpha, rpTNF‑ alpha, and rhTNF‑ beta. In Western blots, this antibody shows 100% cross-reactivity with rmTNF‑ alpha and no cross-reactivity with rfeTNF‑ alpha.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 10-40 µg/mL in the presence of 0.025 ng/mL Recombinant Rat TNF‑ alpha.
来源	Monoclonal Mouse IgG₁ Clone # 45418
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNFalpha in vitro. Authors: Cheng H, Davis D, Hasheminassab S, Sioutas C, Morgan T, Finch C J Neuroinflammation, 2016;13(1):19. Species: Rat Sample Type: Whole Cells Application: Neut Beneficial Effect of Moderate Exercise in Kidney of Rat after Chronic Consumption of Cola Drinks Authors: G Cao, J González, A Müller, G Ottaviano, G Ambrosio, JE Toblli, J Milei PLoS ONE, 2016;11(3):e0152461. Species: Rat Sample Type: Whole Tissue Application: IHC - Paraffin embedded Neuronal synapse formation induced by microglia and interleukin 10. Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J PLoS ONE, 2013;8(11):e81218. Species: Rat Sample Type: Whole Cells Application: Neut Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation. BMC Neurosci, 2012;13(0):50. Species: Rat Sample Type: Tissue Homogenates Application: WB Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology. Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA Infect. Immun., 2008;76(8):3577-86. Species: Rat Sample Type: BALF Application: ELISA Development In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. Authors: Roche M, Diamond M, Finn DP J. Neuroimmunol., 2006;181(1):57-67. Species: Rat Sample Type: Plasma Application: ELISA Development Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNF-alpha. Authors: Nagakawa Y, Williams GM, Zheng Q, Tsuchida A, Aoki T, Montgomery RA, Klein AS, Sun Z Hepatology, 2005;42(1):208-15. Species: Rat Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant rat TNF-alpha Accession # P16599
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Estler, H.C. et al. (1992) Biol. Chem. Hoppe-Seyler 373:271. Tang, P. et al. (1996) Biochemistry 35:8216. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. Romanatto, T. et al. (2007) Peptides 28:1050. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rat TNF‑ alpha Antibody. Recombinant Rat TNF‑ alpha (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF‑ alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF‑ alpha Monoclonal Antibody (Catalog # MAB510). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).