Human Leptin MAb (Clone 44802) (500 UG)【科瑞奥博】

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Human Leptin MAb (Clone 44802) (500 UG)

货号： MAB398-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB398-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB398；Detection antibody：BAM398; and protein: 398-LP-01M
别名	FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.5-2.0 µg/mL ) ELISA Standard ( )
目标/特异性	Captures human Leptin/OB in sandwich ELISAs. In this format, this antibody shows less than 0.1% cross-reactivity with recombinant mouse (rm) Leptin/OB and rrLeptin/OB.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND₅₀) is typically 0.4-1.6 µg/mL in the presence of 7.5 ng/mL Recombinant Human Leptin/OB.
来源	Monoclonal Mouse IgG₁ Clone # 44802
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3952 (Human); 16846 (Mouse); 25608 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Regulatory T cell proliferative potential is impaired in human autoimmune disease. Authors: Carbone F, De Rosa V, Carrieri P, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G Nat Med, 2014;20(1):69-74. Species: Human Sample Type: Whole Cells Application: Neut Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK J. Immunol. Methods, 2012;378(1):72-80. Species: Human Sample Type: Serum Application: ELISA Development A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects. Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B Metab. Clin. Exp., 2009;58(7):946-53. Species: Human Sample Type: Serum Application: Luminex Assay Development Leptin, blood pressure, and aerobic capacity in women. Authors: Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG Am. J. Hypertens., 2008;21(11):1245-50. Species: Human Sample Type: Serum Application: ELISA Development Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production. Authors: Emsley HC, Smith CJ, Gavin CM BMC Neurol, 2007;7(0):5. Species: Human Sample Type: Plasma Application: ELISA Development Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis. Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA J. Assist. Reprod. Genet., 2005;22(6):257-64. Species: Human Sample Type: Follicular Fluid Application: ELISA Development Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes. Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H Eur. Cytokine Netw., 2002;13(2):225-9. Species: Human Sample Type: Plasma Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human Leptin/OB Val22-Cys167 Accession # P41159
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Considine, R. and J. Caro (1996) Clinical Chemistry 42:843. Tartaglia, L.A. et al. (1995) Cell 83:1263. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

参考图片

Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin/OB Monoclonal Antibody (Catalog # MAB398). The ND₅₀ is typically 0.4-1.6 µg/mL.