Human CXCR1/IL-8 RA MAb (Clone 42705) (500 UG)【科瑞奥博】

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Human CXCR1/IL-8 RA MAb (Clone 42705) (500 UG)

货号： MAB330-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB330-500
简介	Clone 42705 was used by HLDA to establish CD designation
别名	CD128; CD181 antigen; CD181; CDw128aC-C; chemokine (C-X-C motif) receptor 1; CKR-1; CMKAR1; C-X-C chemokine receptor type 1; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 receptor type 1; IL-8R A; IL8R1; IL8RA; IL8RAC-C-CKR-1; IL8RBA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A
全称	Interleukin 8 Receptor A
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR1. The Neutralization Dose (ND₅₀) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL‑8.
来源	Monoclonal Mouse IgG_2A Clone # 42705
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3577 (Human); 227288 (Mouse); 54258 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CXCL1/CXCL8 (GROalpha/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro. Authors: Omatsu T, Cepinskas G, Clarson C, Patterson E, Alharfi I, Summers K, Couraud P, Romero I, Weksler B, Fraser D Am J Physiol Endocrinol Metab, 2014;306(9):E1077-84. Species: Human Sample Type: Whole Cells Application: Neut The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells. Authors: Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T J Biol Chem, 2014;289(14):10029-44. Species: Human Sample Type: Whole Cells Application: Neut Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche. Authors: Infanger, David W, Cho, YouJin, Lopez, Brina S, Mohanan, Sunish, Liu, S Chris, Gursel, Demirkan, Boockvar, John A, Fischbach, Claudia Cancer Res, 2013;73(23):7079-89. Species: Human Sample Type: Whole Cells Application: Functional Assay Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Nat. Med., 2011;17(11):1498-503. Species: Human Sample Type: In Vivo Application: In Vivo Homing Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6. Authors: Eikawa S, Ohue Y, Kitaoka K J. Immunol., 2010;185(11):6734-40. Species: Human Sample Type: Whole Cells Application: Neut Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients. Authors: Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A Immunol. Lett., 2010;130(1):97-106. Species: Human Sample Type: Whole Cells Application: Flow CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion. Authors: Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK Br. J. Cancer, 2009;100(10):1638-46. Species: Human Sample Type: Cell Lysates Application: WB Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis. Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P Clin. Exp. Immunol., 2009;158(1):143-53. Species: Human Sample Type: Whole Cells Application: Neut Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Authors: Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, ONeal W, Sorscher EJ, Abraham E, Blalock JE Am. J. Respir. Crit. Care Med., 2008;178(8):822-31. Species: Human Sample Type: Whole Cells Application: Neut A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation. Authors: Gaggar A, Jackson PL, Noerager BD, OReilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE J. Immunol., 2008;180(8):5662-9. Species: Mouse Sample Type: Whole Cells Application: Neut Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina. Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89. Species: Human Sample Type: Whole Tissue Application: IHC Fresh Shear-induced capping of L-selectin on the neutrophil surface during centrifugation. Authors: Lee D, King MR J. Immunol. Methods, 2007;328(1):97-105. Species: Human Sample Type: Whole Cells Application: ICC Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression. Authors: Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA Leuk. Res., 2007;31(5):591-8. Species: Human Sample Type: Whole Cells Application: Flow Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells. Authors: Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR Crit. Care Med., 2007;35(3):879-85. Species: Human Sample Type: Whole Cells Application: Neut Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. Authors: Smith C, Damas JK, Otterdal K, ÃƒËœIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P J. Am. Coll. Cardiol., 2006;48(8):1591-9. Species: Human Sample Type: Whole Cells Application: Neut Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: Whole Cells Application: Neut IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Cell Culture Supernates Application: Neut Chemokine receptor expression in rat adjuvant-induced arthritis. Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE Arthritis Rheum., 2005;52(12):3718-30. Species: Rat Sample Type: Whole Tissue Application: IHC The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium. Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M J. Neuroimmunol., 2005;161(1):49-60. Species: Guinea Pig Sample Type: Whole Cells Application: ICC Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, RÃƒÂ©vÃƒÂ©sz T Blood, 2003;103(7):2806-8. Species: Human Sample Type: Whole Cells Application: Flow Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma. Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;apos;Reilly RJ, Dupont B, Vyas YM J. Immunol., 2003;171(12):6891-9. Species: Human Sample Type: Whole Cells Application: Flow Expression of functional CCR and CXCR chemokine receptors in podocytes. Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H J. Immunol., 2002;168(12):6244-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RA Met1-Leu350 Accession # AAA59159
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Cancer Stem Cell Markers Chemokine Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors Dendritic Cells: A New Immunotherapy? Human CD Marker Products Macrophage Activation Markers Megakaryocytes Mini-review: Multiple Sclerosis: Immune Cell Access to the Central Nervous System Monocyte Markers Neuroinflammation Neutrophils T Cell Migration/Adhesion

参考图片

Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human
CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

CXCR1/IL‑8 RA in Human Endometrial Cancer Tissue. CXCR1/IL‑8 RA was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

CXCR1/IL‑8 RA in Human Lymph Nodes. CXCR1/IL‑8 RA was detected in immersion fixed frozen sections of human lymph nodes using Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to lymphocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody. Recombinant Human CXCL8/IL‑8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL‑8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330). The ND₅₀ is typically 0.4‑2.0 µg/mL.