Mouse CXCL2/MIP-2 MAb (Clone 40605) (500 UG)【科瑞奥博】

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Mouse CXCL2/MIP-2 MAb (Clone 40605) (500 UG)

货号： MAB452-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB452-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB452；Detection antibody：BAF452; and protein: 452-M2-010
别名	chemokine (C-X-C motif) ligand 2; CINC-2a; CINC-3; C-X-C Motif Chemokine 2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CXCL2 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta, rhCXCL3/GRO gamma, or recombinant mouse CXCL1/KC.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.
来源	Monoclonal Rat IgG_2B Clone # 40605
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2920 (Human); 20310 (Mouse); 114105 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Patrolling monocytes promote intravascular neutrophil activation and glomerular injury in the acutely inflamed glomerulus Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. Authors: Roy M, Richard JF, Dumas A, Vallieres L J Neuroinflammation, 2012;9(0):18. Species: Mouse Sample Type: In Vivo Application: In vivo Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH J. Clin. Invest., 2011;121(11):4446-61. Species: Mouse Sample Type: In Vivo Application: In vivo Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Am. J. Pathol., 2011;179(6):2730-9. Species: Mouse Sample Type: BALF Application: ELISA Development TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines. Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG J. Lipid Res., 2011;52(7):1307-18. Species: Mouse Sample Type: In Vivo Application: In vivo Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease. Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM J. Immunol., 2010;184(8):4159-69. Species: Mouse Sample Type: In Vivo Application: In vivo Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis. Authors: Chou RC, Kim ND, Sadik CD Immunity, 2010;33(2):266-78. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils. Authors: Massena S, Christoffersson G, Hjertstrom E, Zcharia E, Vlodavsky I, Ausmees N, Rolny C, Li JP, Phillipson M Blood, 2010;116(11):1924-31. Species: Mouse Sample Type: In vivo localization Application: IHC Fresh Intrinsic renal cell and leukocyte-derived TLR4 aggravate experimental anti-MPO glomerulonephritis. Authors: Summers SA, van der Veen BS, OSullivan KM Kidney Int., 2010;78(12):1263-74. Species: Mouse Sample Type: In Vivo Application: In vivo ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T Microbes Infect., 2009;11(4):524-30. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA J. Biol. Chem., 2009;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling. Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM Blood, 2008;111(7):3343-54. Species: Mouse Sample Type: In Vivo Application: In vivo Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways. Authors: De Filippo K, Henderson RB, Laschinger M, Hogg N J. Immunol., 2008;180(6):4308-15. Species: Mouse Sample Type: In Vivo Application: In vivo Critical role of CD44 in hepatotoxin-mediated liver injury. Authors: Kimura K, Nagaki M, Kakimi K, Saio M, Saeki T, Okuda Y, Kuwata K, Moriwaki H J. Hepatol., 2008;48(6):952-61. Species: Mouse Sample Type: In Vivo Application: In vivo CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T Immunology, 2008;125(2):272-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Type II NKT cell-mediated anergy induction in type I NKT cells prevents inflammatory liver disease. Authors: Halder RC, Aguilera C, Maricic I, Kumar V J. Clin. Invest., 2007;117(8):2302-12. Species: Mouse Sample Type: In Vivo Application: In vivo The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM Blood, 2007;111(1):42-9. Species: Mouse Sample Type: In Vivo Application: In vivo Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Infect. Immun., 2006;74(12):6730-8. Species: Mouse Sample Type: BALF Application: ELISA Development Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis. Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A J. Immunol., 2006;177(12):8650-7. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide. Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO J. Invest. Dermatol., 2006;126(8):1792-803. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ Eur. J. Immunol., 2006;36(8):2025-34. Species: Mouse Sample Type: Peritoneal Exudate Application: ELISA Development Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA J. Immunol., 2006;177(1):612-20. Species: Mouse Sample Type: BALF Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation. Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD J. Immunol., 2005;175(4):2589-97. Species: Mouse Sample Type: BALF Application: ELISA Development Transcriptional profiling of lipopolysaccharide-induced acute lung injury. Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS Infect. Immun., 2004;72(12):7247-56. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways. Authors: Miyamoto M, Prause O, Sjostrand M, Laan M, Lotvall J, Linden A J. Immunol., 2003;170(9):4665-72. Species: Mouse Sample Type: In Vivo Application: In vivo Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation. Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP J. Immunol., 2001;166(2):1233-40. Species: Mouse Sample Type: In Vivo Application: In vivo Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function. Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S Stem Cells Transl Med, 0;4(1):44-55. Species: Human Sample Type: Whole Cells Application: Bioassay Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C Immunity, 0;43(5):945-58. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Capture
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse CXCL2/GRO beta /MIP-2/CINC-3 Ala28-Asn100 Accession # P10889
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines. MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chemokines and Receptors in Angiogenesis CXC Chemokines and Receptors ICC / IHC Images: CXCL2/GRO beta/MIP-2/CINC-3 Inflammatory Mediators Macrophage Activation Markers Neuroinflammation

参考图片

Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCL2/MIP‑2 Antibody. Recombinant Mouse CXCL2/MIP‑2 (Catalog #
452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP‑2 Monoclonal Antibody (Catalog # MAB452). The ND₅₀ is typically 1-4 µg/mL.