| 货号 | MAB608-500 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：MAB608-500；Detection antibody：BAF308; and protein: 308-FKN-005 |
| 别名 | FL; Flt3 ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine | 全称 | fms-like Tyrosine Kinase 3 Ligand |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects human Flt-3 Ligand in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 5 ng/mL Recombinant Human Flt‑3 Ligand. |
| 来源 | Monoclonal Mouse IgG1 Clone # 40416 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2323 (Human); 14256 (Mouse); 493796 (Feline) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand Thr27-Pro185 Accession # AAA17999 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody. Recombinant Human Flt‑3 Ligand (Catalog # 308-FK) stimulates proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt‑3 Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt‑3 Ligand Monoclonal Antibody (Catalog # MAB608). The ND50 is typically 0.02‑0.06 µg/mL. |
