Mouse IL-13 MAb (Clone 38213) (500 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Mouse IL-13 MAb (Clone 38213) (500 UG)

货号： MAB413-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB413-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB413；Detection antibody：BAF413; and protein: 413-ML-005
别名	ALRHMGC116789; BHR1interleukin-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600
全称	Interleukin 13
反应种属	Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse IL-13 in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human IL‑13 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.3-0.8 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.
来源	Monoclonal Rat IgG_2A Clone # 38213
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis Authors: SR Damle, RK Martin, JV Cross, DH Conrad Mucosal Immunol, 2016;0(0):. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Capture RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets. Authors: Knudson C, Hartwig S, Meyerholz D, Varga S PLoS Pathog, 2015;11(3):e1004757. Species: Mouse Sample Type: cell supernates Application: ELISA Capture CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome. Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C Mucosal Immunol, 2014;7(2):417-27. Species: Mouse Sample Type: Whole Cells Application: ELISA Capture Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease. Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM J. Immunol., 2010;184(8):4159-69. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development. Authors: Mosconi E, Rekima A, Seitz-Polski B Mucosal Immunol, 2010;3(5):461-74. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development IL-6 is required for airway mucus production induced by inhaled fungal allergens. Authors: Neveu WA, Allard JB, Dienz O, Wargo MJ, Ciliberto G, Whittaker LA, Rincon M J. Immunol., 2009;183(3):1732-8. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions. Authors: Daniel C, Sartory NA, Zahn N Mol. Immunol., 2007;44(13):3305-16. Species: Mouse Sample Type: In Vivo Application: In vivo Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia. Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(5):L862-70. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL Am. J. Pathol., 2006;168(6):1940-50. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Authors: Schmitz J, Owyang A, Oldham E, Song Y, Murphy E, McClanahan TK, Zurawski G, Moshrefi M, Qin J, Li X, Gorman DM, Bazan JF, Kastelein RA Immunity, 2005;23(5):479-90. Species: Mouse Sample Type: Serum Application: ELISA Development Persistence of bronchopulmonary hyper-reactivity and eosinophilic lung inflammation after anti-IL-5 or -IL-13 treatment in allergic BALB/c and IL-4Ralpha knockout mice. Authors: Proust B, Nahori MA, Ruffie C, Lefort J, Vargaftig BB Clin. Exp. Allergy, 2003;33(1):119-31. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse IL-13 Accession # P20109
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Wills-Karp, M. (2004) Immunol. Rev. 202:175. Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265. Brown, K.D. et al. (1989) J. Immunol. 142:679. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231. Ruetten, H. and C. Thiemermann (1997) Shock 8:409. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464. Tabata, Y. et al. (2007) J. Immunol. 177:7905. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479. Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

参考图片

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413). The ND₅₀ is typically 0.3-0.8 µg/mL.