Mouse IFN-gamma MAb (Clone 37895) (500 UG)【科瑞奥博】

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Mouse IFN-gamma MAb (Clone 37895) (500 UG)

货号： MAB485-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB485-500
别名	IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
全称	Interferon gamma
反应种属	Mouse
应用	Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN‑ gamma, rrIFN‑ gamma, rpIFN‑ gamma, rrmIFN‑ gamma, rfeIFN‑ gamma, or rcaIFN‑ gamma.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND₅₀) is typically 0.075-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IFN‑ gamma.
来源	Monoclonal Rat IgG_2A Clone # 37895
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia. Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G J. Immunol., 2011;186(9):5435-42. Species: Mouse Sample Type: In Vivo Application: In vivo The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function. Authors: Jin W, Qi S, Luo H BMC Immunol., 2011;12(0):68. Species: Mouse Sample Type: Whole Cells Application: Neut Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA FASEB J., 2011;25(1):409-19. Species: Mouse Sample Type: Whole Cells Application: Neut IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma. Authors: Deepak P, Kumar S, Kishore D, Acharya A Int. Immunol., 2010;22(1):53-63. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease. Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM J. Immunol., 2010;184(8):4159-69. Species: Mouse Sample Type: In Vivo Application: In vivo TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice. Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK J. Immunol., 2008;181(6):4089-97. Species: Mouse Sample Type: Whole Cells Application: Neut Crucial role of the interleukin-6/interleukin-17 cytokine axis in the induction of arthritis by glucose-6-phosphate isomerase. Authors: Iwanami K, Matsumoto I, Tanaka-Watanabe Y, Inoue A, Mihara M, Ohsugi Y, Mamura M, Goto D, Ito S, Tsutsumi A, Kishimoto T, Sumida T Arthritis Rheum., 2008;58(3):754-63. Species: Mouse Sample Type: In Vivo Application: In vivo Mitochondrial structural changes and dysfunction are associated with experimental allergic asthma. Authors: Mabalirajan U, Dinda AK, Kumar S, Roshan R, Gupta P, Sharma SK, Ghosh B J. Immunol., 2008;181(5):3540-8. Species: Mouse Sample Type: In Vivo Application: In vivo Controlled delivery of T-box21 small interfering RNA ameliorates autoimmune alopecia (Alopecia Areata) in a C3H/HeJ mouse model. Authors: Nakamura M, Jo J, Tabata Y, Ishikawa O Am. J. Pathol., 2008;172(3):650-8. Species: Mouse Sample Type: In Vivo Application: In vivo Maternal immune activation alters fetal brain development through interleukin-6. Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH J. Neurosci., 2007;27(40):10695-702. Species: Mouse Sample Type: In Vivo Application: In vivo A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model. Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M J. Mol. Cell. Cardiol., 2006;41(5):885-92. Species: Mouse Sample Type: Whole Tissue Application: IHC Dendritic cells and NK cells stimulate bystander T cell activation in response to TLR agonists through secretion of IFN-alpha beta and IFN-gamma. Authors: Kamath AT, Sheasby CE, Tough DF J. Immunol., 2005;174(2):767-76. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse IFN-gamma
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype pro-inflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque IFN-gamma. IFN-gamma dimers bind to IFN‑ gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits anti-viral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Gray, P.W. and D.V. Goeddel (1983) Proc. Natl. Acad. Sci. USA 80:5842. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

参考图片

Detection of IFN‑ gamma in Mouse Splenoctyes by Flow Cytometry. Mouse splenocyteseither (A) stimulated to induce Th1 cells or
(B) unstimulated were stained with Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

IFN‑ gamma Inhibition of
EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma Antibody. Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB485). The ND₅₀ is typically 0.075-0.3 µg/mL.