Human IL-10 R alpha MAb (Clone 37607) (500 UG)【科瑞奥博】

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Human IL-10 R alpha MAb (Clone 37607) (500 UG)

货号： MAB274-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB274-500
别名	CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10RA; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha
全称	Interleukin 10 Receptor alpha
反应种属	Human
应用	Neutralization
目标/特异性	Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL‑10 R alpha.
使用方法	Neutralization: Measured by its ability to neutralize IL‑10 R alpha -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND₅₀) is typically 0.03-0.1 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.
来源	Monoclonal Mouse IgG₁ Clone # 37607
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3587 (Human); 16154 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages Sci Rep, 2016;6(0):31959. Species: Human Sample Type: Whole Cells Application: Blocking Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms. Authors: von Lanzenauer S, Wolk K, Hoflich C, Kunz S, Grunberg B, Docke W, Reineke U, Asadullah K, Sterry W, Volk H, Sabat R Genes Immun, 2015;16(1):8-14. Species: Human Sample Type: Whole Cells Application: Flow Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection. Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B J. Virol., 2008;82(7):3736-50. Species: Human Sample Type: Whole Cells Application: Neut Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. Authors: Chang WL, Baumgarth N, Yu D, Barry PA J. Virol., 2004;78(16):8720-31. Species: Human Sample Type: Whole Cells Application: Neut In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures. Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB Blood, 2004;104(2):453-61. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha His22-Asn235 Accession # Q13651.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Development and Differentiation Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function Human CD Marker Products ICC / IHC Images: IL-10 R alpha IL-10/Interferon Family Monocyte Markers Neuroinflammation Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs)

参考图片

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R alpha Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 R alpha Monoclonal Antibody (Catalog # MAB274). The ND₅₀ is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL).