Human B7-2/CD86 MAb (Clone 37301) (500 UG)【科瑞奥博】

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Human B7-2/CD86 MAb (Clone 37301) (500 UG)

货号： MAB141-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB141-500
别名	Activation B7-2 antigen; B70; B7-2 antigen; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86
反应种属	Human
应用	Western Blot(5 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.
使用方法	Western Blot: 5 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize B7‑2/CD86-induced IL-2 secretion in the Jurkat human acute T cell leukemia cell line. Freeman, G.J. et al. (1993) Science 262:909. The Neutralization Dose (ND₅₀) is typically 0.5-2.5 μg/mL in the presence of 2 μg/mL Recombinant Human B7‑2/CD86 Fc Chimera.
来源	Monoclonal Mouse IgG₁ Clone # 37301
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	942 (Human); 12524 (Mouse); 56822 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor. Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z Cancer Res, 2012;72(22):5733-43. Species: Human Sample Type: Whole Cells Application: ICC Fresh ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 2012;119(26):6268-77. Species: Human Sample Type: Whole Cells Application: Neut Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells. Authors: Tso GH, Law HK, Tu W Stem Cells, 2010;28(5):939-54. Species: Human Sample Type: Whole Cells Application: Neut Critical role of lipid rafts in CD154-mediated T cell signaling. Authors: El Fakhry Y, Alturaihi H, Diallo D, Merhi Y, Mourad W Eur. J. Immunol., 2010;40(3):770-9. Species: Human Sample Type: Whole Cells Application: Flow Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells. Authors: van der Vliet HJ, Wang R, Yue SC, Koon HB, Balk SP, Exley MA J. Immunol., 2008;180(11):7287-93. Species: Human Sample Type: Whole Cells Application: Neut The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. Authors: Sakthivel P, Shively V, Kakoulidou M J. Intern. Med., 2007;261(4):399-407. Species: Human Sample Type: Plasma Application: ELISA Development Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients. Authors: Hock BD, Patton WN, Budhia S, Mannari D, Roberts P, McKenzie JL Leukemia, 2002;16(5):865-73. Species: Human Sample Type: Plasma Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86 Ala23-His244 Accession # P42081
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Azuma, M.et al. (1993) Nature 366:76. Freeman, G.J.et al. (1993) Science 262:909. Freeman, G.et al. (1991) J. Exp. Med. 174:625. Selvakumar, A.et al. (1993) Immunogenetics 38:292. Chen, C.et al. (1994) J. Immunol. 152:4929. Freeman, G.J.et al. (1993) J. Exp. Med. 178:2185.

参考图片

Detection of Human B7‑2/CD86 by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for B7‑2/CD86 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of B7‑2/CD86 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Cell IL-2 Secretion Induced by B7‑2/CD86 and Neutralization by Human B7‑2/CD86 Antibody. Recombinant Human B7‑2/CD86 Fc Chimera induces IL‑2 secretion in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine kit (Catalog # D2050). Under these conditions, IL-2 secretion elicited by B7‑2/
CD86 is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑2/CD86 Monoclonal Antibody (Catalog # MAB141). The ND₅₀ is typically 0.5-2.5 μg/mL.