Human/Mouse BMP-9 MAb (Clone 360107) (500 UG)【科瑞奥博】

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Human/Mouse BMP-9 MAb (Clone 360107) (500 UG)

货号： MAB3209-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB3209-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB3209-500；Detection antibody：BAF3209; and protein: 3209-BP-010
别名	BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2
全称	Bone Morphogenetic Protein 9
反应种属	Human/Mouse
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human and primate BMP‑9 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP‑1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) BMP‑9 and 10% cross‑reactivity with rmBMP-10 is observed.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND₅₀) is typically 0.01-0.04 µg/mL in the presence of 2 ng/mL Recombinant Human BMP‑9.
来源	Monoclonal Mouse IgG_2B Clone # 360107
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2658 (Human); 12165 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs. Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing Nat Genet, 2013;45(1):34-42. Species: Mouse Sample Type: whole cells Application: Neut BMP9 and BMP10 are critical for postnatal retinal vascular remodeling. Blood, 2012;119(25):6162-71. Species: Mouse Sample Type: In Vivo Application: In vivo Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium. Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI Blood, 2012;119(21):5037-47. Species: Human Sample Type: Cell Lysate Application: IP Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting. Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P J. Biol. Chem., 2012;287(22):18551-61. Species: Human Sample Type: Whole Cells Application: Neut A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum. Authors: Herrera B, Inman GJ, BMC Cell Biol., 2009;10(0):20. Species: Human Sample Type: recombinant BMP-9 Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human BMP-9 Ser320-Arg429 Accession # Q9UK05
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Mouse, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Chen, D. et al. (2004) Growth Factors 22:233. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111. Song, J.J. et al. (1995) Endocrinology 136:4293. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937. Chen, C. et al. (2003) Nat. Biotechnol. 21:294. Lopez-Coviella, I. et al. (2000) Science 289:313. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6984. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611. Ploemacher, R.E. et al. (1999) Leukemia 13:428.

参考图片

Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Recombinant Human BMP‑9 (Catalog # 3209‑BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse/Primate BMP‑9 Monoclonal Antibody (Catalog # MAB3209). The ND₅₀ is typically 0.01-0.04 µg/mL.