Human Thrombopoietin MAb (Clone 34817) (500 UG)【科瑞奥博】

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Human Thrombopoietin MAb (Clone 34817) (500 UG)

货号： MAB288-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB288-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB288；Detection antibody：BAM288 or BAF288; and protein: 288-TPN-025
别名	Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; megakaryocyte stimulating factor; MGDF; MGDFC-mpl ligand; MK-CSF; ML; MPL ligand; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene ligand; THPO; thrombopoietin nirs variant 1; thrombopoietin; Tpo; TPOMKCSF
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human Thrombopoietin/Tpo in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) G-CSF, rhG-CSF R, rhGM-CSF, recombinant mouse GM-CSF, or rhM-CSF is observed.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Thrombopoietin/Tpo-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 5‑10 µg/mL in the presence of 1.5 ng/mL Recombinant Human Thrombopoietin/Tpo.
来源	Monoclonal Mouse IgG_2B Clone # 34817
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7066 (Human); 21832 (Mouse); 81811 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A multiplex immunoassay for human adipokine profiling. Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E Clin. Chem., 2010;56(0):1320. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development
纯化方式	Protein A or G purified from ascites
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Thrombopoietin/Tpo Ser22-Gly353 Accession # P40225
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Tpo is a secreted protein that regulates megakaryocytopoiesis andthrombopoiesis. It binds to and induces signaling through the Tporeceptor, which is encoded by the c-Mplprotooncogene.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Megakaryocytes Mini-review: Thrombopoietin (Tpo) Myeloid Lineage Markers Osteoblast and Osteoclast Markers Osteogenesis Markers Osteoprogenitor Markers

参考图片

Cell Proliferation Induced by Thrombopoietin and Neutralization by Human Thrombopoietin Antibody. Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Thrombopoietin/Tpo (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Thrombopoietin/Tpo Monoclonal Antibody (Catalog # MAB288). The ND₅₀ is typically 5‑10 µg/mL.