Human IL-15 MAb (Clone 34505) (500 UG)【科瑞奥博】

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Human IL-15 MAb (Clone 34505) (500 UG)

货号： MAB647-500 基本售价： 10360.6 元规格： 500 ug

产品信息

概述
货号	MAB647-500
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB647；Detection antibody：BAM247; and protein: 247-IL-005
别名	IL-15MGC9721; interleukin 15; interleukin-15
全称	Interleukin 15
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.5-2.0 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human IL-15 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, -1 beta, -2, -3, -4, -5, -6, -7, -8, -9, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13 is observed.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 4-20 ng/mL in the presence of 3 ng/mL Recombinant Human IL‑15.
来源	Monoclonal Mouse IgG₁ Clone # 34505
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. Authors: Pfleger C, Schloot N, ter Veld F J. Immunol. Methods, 2007;329(1):214-8. Species: Human Sample Type: Serum Application: Luminex Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns. Authors: Rhoton-Vlasak A, Hardt N Fertil. Steril., 2005;83(3):659-65. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23. Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB Exp. Dermatol., 2004;13(12):764-72. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling. Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M Leuk. Res., 2004;28(10):1023-31. Species: Human Sample Type: Cell Lysates Application: WB NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs. Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L Blood, 2004;104(10):3267-75. Species: Human Sample Type: Whole Cells Application: ICC Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor. Authors: Searle RF, Bromage SJ, Palmer J, Curry JE, Lang AK Immunology, 2000;99(3):411-7. Species: Human Sample Type: Amniotic Fluid Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL-15 Asn49-Ser162 Accession # P40933
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8⁺ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Grabstein, K. et al. (1994) Science 264:965. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444. Giri, J.G. et al. (1995) EMBO 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851. Dubois, S. et al. (2002) Immunity 17:537. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

参考图片

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) induces proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑15 Monoclonal Antibody (Catalog # MAB647). The ND₅₀ is typically 4-20 ng/mL.