Human CXCR4 (Fusin) MAb (Clone 12G5) (500 UG)【科瑞奥博】

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Human CXCR4 (Fusin) MAb (Clone 12G5) (500 UG)

货号： MAB170 基本售价： 5998.2 元规格： 500 ug

产品信息

概述
货号	MAB170
别名	CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
反应种属	Human
应用	Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human CXCR4.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND₅₀) is typically 0.3-1.2 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.
来源	Monoclonal Mouse IgG_2A Clone # 12G5
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4 Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley J Biol Chem, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Bioassay Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: IHC Fresh HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1? Mol Cell Biol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Neut Ctrl Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia. Authors: Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C Cancer Res, 2015;75(19):4153-63. Species: Human Sample Type: Whole Cells Application: Flow Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines. Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y PLoS ONE, 2015;10(3):e0120069. Species: Human Sample Type: Whole Cells Application: Neut An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration. Authors: Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B J Biol Chem, 2013;288(40):28656-67. Species: Human Sample Type: Whole Cells Application: Neut The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S Clin. Cancer Res., 2011;17(11):3619-30. Species: Human Sample Type: Whole Cells Application: Neut Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D J. Immunol., 2008;181(11):8053-67. Species: Human Sample Type: Whole Cells Application: Flow Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Authors: Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Exp. Hematol., 2008;36(9):1121-31. Species: Human Sample Type: Whole Cells Application: Neut Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed. Authors: McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S Stem Cells, 2007;25(1):29-38. Species: Human Sample Type: Whole Cells Application: Flow Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells. Authors: Kaminski A, Ma N, Donndorf P, Lindenblatt N, Feldmeier G, Ong LL, Furlani D, Skrabal CA, Liebold A, Vollmar B, Steinhoff G Lab. Invest., 2007;88(1):58-69. Species: Mouse Sample Type: In Vivo Application: In vivo Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium. Authors: Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW Cancer Res., 2007;67(9):4244-53. Species: Human Sample Type: Whole Cells Application: Neut Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A J. Cell. Physiol., 2006;207(2):364-73. Species: Human Sample Type: Whole Cells Application: ICC CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes. Authors: Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M AIDS Res. Hum. Retroviruses, 2006;22(11):1152-61. Species: Human Sample Type: Whole Cells Application: Neut Skin homing of Sezary cells involves SDF-1-CXCR4 signaling and down-regulation of CD26/dipeptidylpeptidase IV. Authors: Narducci MG, Scala E, Bresin A, Caprini E, Picchio MC, Remotti D, Ragone G, Nasorri F, Frontani M, Arcelli D, Volinia S, Lombardo GA, Baliva G, Napolitano M, Russo G Blood, 2005;107(3):1108-15. Species: Human Sample Type: Whole Tissue Application: IHC Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells. Authors: Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F Stem Cells, 2004;22(6):1015-29. Species: Human Sample Type: Whole Cells Application: Flow CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice. Authors: Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T Cancer Res., 2004;64(8):2817-24. Species: Human Sample Type: Whole Cells Application: ICC Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells. Authors: Lawrence DM, Durham LC, Schwartz L, Seth P, Maric D, Major EO J. Virol., 2004;78(14):7319-28. Species: Human Sample Type: Whole Cells Application: Flow Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells. Authors: Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schroder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG J. Leukoc. Biol., 2002;72(4):837-45. Species: Human Sample Type: Whole Cells Application: ICC Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia? Authors: Schneider P, Vasse M, Al Bayati A, Lenormand B, Vannier JP Br. J. Haematol., 2002;119(2):579-80. Species: Human Sample Type: Whole Cells Application: ICC CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor. Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE Blood, 2002;100(7):2321-9. Species: Human Sample Type: Whole Cells Application: Flow Expression of functional CCR and CXCR chemokine receptors in podocytes. Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H J. Immunol., 2002;168(12):6244-52. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function. Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S Stem Cells Transl Med, 0;4(1):44-55. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from ascites
免疫原	CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line Accession # P61073
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Endres, M.J.et al. (1996) Cell 87:745.

参考图片

Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/ Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND₅₀ is typically 0.3-1.2 µg/mL.