Mouse CCL27/CTACK MAb (Clone 68623) (500 UG)【科瑞奥博】

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Mouse CCL27/CTACK MAb (Clone 68623) (500 UG)

货号： MAB725 基本售价： 5175.3 元规格： 500 ug

产品信息

概述
货号	MAB725
描述	For ELISA the Antibody Pairs information：Capture antibody：MAB725；Detection antibody：BAF725; and protein: 725-CK-025
别名	ALP; C-C motif chemokine 27; chemokine (C-C motif) ligand 27; CTACK; CTACKSmall-inducible cytokine A27; CTAK; cutaneous T-cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESKINE; IL-11 Ralpha-locus chemokine; ILC; ILCCC chemokine ILC; PESKY; SCYA27IL-11 R-alpha-locus chemokine; skinkine; small inducible cytokine subfamily A (Cys-Cys), member 27
反应种属	Mouse
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse CCL27/CTACK in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant cottonrat CCL5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18,19, 20, 21, 22, 23, 25, 27, 28, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, orrecombinant rat CCL20.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL27/CTACK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR10. The Neutralization Dose (ND₅₀) is typically 2.5-10 µg/mL in the presence of 1 µg/mL Recombinant Human CCL27/CTACK.
来源	Monoclonal Rat IgG_2B Clone # 68623
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10850 (Human); 20301 (Mouse); 362505 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia. Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G J. Immunol., 2011;186(9):5435-42. Species: Mouse Sample Type: In Vivo Application: In vivo CCL27-transgenic mice show enhanced contact hypersensitivity to Th2, but not Th1 stimuli. Authors: Kagami S, Saeki H, Tsunemi Y, Nakamura K, Kuwano Y, Komine M, Nakayama T, Yoshie O, Tamaki K Eur. J. Immunol., 2008;38(3):647-57. Species: Mouse Sample Type: In Vivo Application: In vivo CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model. Authors: Chen L, Lin SX, Agha-Majzoub R, Overbergh L, Mathieu C, Chan LS Int. Immunol., 2006;18(8):1233-42. Species: Mouse Sample Type: Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human CCL27/CTACK Leu26-Asn120 Accession # NP_035466
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature mouse CCL27 is a 95 amino acid (aa) protein that shares 57% aa and 87% aa sequence identity with human and rat CCL27, respectively (2). It shares 18-31% aa sequence identity with other mouse CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4-7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7-9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10-12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA⁺ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13-15).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Kunkel, L. and E.C. Butcher (2002) Immunity 16:1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 258:737. Gortz, A. et al. (2002) J. Immunol. 169:1387. Morales, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96:14470. Homey, B. et al. (2000) J. Immunol. 164:3465. Homey, B. et al. (2002) Nat. Med. 8:157. Inokuma, D. et al. (2006) Stem Cells 24:2810. Jarmin, D. et al. (2000) J. Immunol. 164:3460. Hirata, T. et al. (2004) J. Biol. Chem. 279:51775. Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551. Reiss, Y. et al. (2001) J. Exp. Med. 194:1541. Soler, D. et al. (2003) Blood 101:1677. Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592. Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334. Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.

参考图片

Chemotaxis Induced by CCL27/CTACK and Neutralization by Mouse CCL27/CTACK Antibody. Recombinant Human CCL27/CTACK (Catalog # 376-CT) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL27/CTACK Monoclonal Antibody (Catalog # MAB725). The ND₅₀ is typically 2.5-10 µg/mL.