| 货号 | MAB65591 |
| 别名 | follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH receptor; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1 | 全称 | Follicle Stimulating Hormone Receptor |
| 反应种属 | Human |
| 应用 | Flow Cytometry,CyTOF-ready,Neutralization |
| 目标/特异性 | Detects human FSH R in direct ELISAs. |
| 使用方法 | Neutralization: Measured by its ability to neutralize FSH alpha / beta -induced cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 1 ng/mL Recombinant Human FSH alpha / beta. |
| 来源 | Monoclonal Mouse IgG2A Clone # 626717 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2492 (Human); 14309 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with human FSH R Accession # M65085 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.5 mg/mL. |
| 背景 | Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
cAMP Production Induced by FSH alpha / beta and Neutralization by Human FSH R Antibody. Recombinant Human FSH alpha / beta (Catalog # 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recombinant Human FSH alpha / beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND50 is typically 0.02-0.1 µg/mL. |
